| HUO Hong-mei,YANG Shun,CHEN Lie-song,LÜ Hai-jun,WANG Ai-dong,ZHANG Li-yuan.Hydrogen-rich saline alleviation on the oxidative stress and early-phase radiation-induced brain injury in rats[J].Chinese Journal of Radiological Medicine and Protection,2012,32(5):485-487 |
| Hydrogen-rich saline alleviation on the oxidative stress and early-phase radiation-induced brain injury in rats |
| Received:March 20, 2012 |
| DOI：10.3760/cma.j.issn.0254-5098.2012.05.008 |
| KeyWords:Hydrogen Irradiation Protection Brain injury Free radical |
| FundProject:江苏省十二五医学重点学科(实验室)资助 |
| Author Name | Affiliation | E-mail | | HUO Hong-mei | The Second Affiliated Hospital of Soochow University, Suzhou 215004, China | | | YANG Shun | The Second Affiliated Hospital of Soochow University, Suzhou 215004, China | | | CHEN Lie-song | The Second Affiliated Hospital of Soochow University, Suzhou 215004, China | | | LÜ Hai-jun | The Second Affiliated Hospital of Soochow University, Suzhou 215004, China | | | WANG Ai-dong | The Second Affiliated Hospital of Soochow University, Suzhou 215004, China | | | ZHANG Li-yuan | The Second Affiliated Hospital of Soochow University, Suzhou 215004, China | zhangliyuan126@126.com |
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| Abstract:: |
| Objective To investigate the effect of hydrogen on radiation-induced acute injury in rat brain.Methods Forty-five mature Sprague-Dawley rats were randomly divided into three groups: saline therapy group, hydrogen therapy group and healthy control group. The whole brain of SD rat was irradiated with single dose of 20 Gy by 4 MeV electrons. Rats in therapy group were injected with hydrogen-rich saline after irradiation and were sacrificed at 1, 3, 7, 14 d post-irradiation. The changes of malonaldehyde (MDA), superoxidase dismutase (SOD) and 8-hydroxydeoxygunosine (8-OHdG) in brain homogenate and the pathological changes in brain hippocampus were observed. Results The brain water content (t=3.78,3.18,P<0.05) and the contents of 8-OHdG (t=2.33, 2.71, 2.33,P<0.05) in the therapy group was lower than the control group at 7 d and 14 d post-irradiation. The contents of SOD were significantly higher(t=2.41-2.92, P<0.05)from 1 to 7 day, while the contents of MDA were significantly lower in therapy group than those in the control group from 1 to 14 day post-irradiation (t=4.01-6.20, P<0.05). Moreover, the damage degree in the nerve cells of hippocampus was less compared to the control group. Conclusions The hydrogen-rich saline could have protection role in irradiation-induced acute brain injury in rats. |
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