| MA Liang,YANG Yang,SHEN Lin-ling,ZHAO Lin,JIAO Yang,XU Jia-ying,FAN Sai-jun.Impact of honokiol on growth and radiosensitivity in nasopharyngeal carcinoma cells[J].Chinese Journal of Radiological Medicine and Protection,2012,32(5):469-474 |
| Impact of honokiol on growth and radiosensitivity in nasopharyngeal carcinoma cells |
| Received:March 20, 2012 |
| DOI：10.3760/cma.j.issn.0254-5098.2012.05.005 |
| KeyWords:Honokiol Nasopharyngeal carcinoma Apoptosis Cell cycle Radiosensitivity |
| FundProject:国家科学自然研究基金面上项目(81071906;81172127);教育部"长江学者和创新团队发展计划资助"项目(IRT0849);苏州市肿瘤放射生物学重点实验室(SZS0802) |
| Author Name | Affiliation | E-mail | | MA Liang | School of Radiation Medicine and Protection, Soochow University Medical College, Suzhou 215123, China | | | YANG Yang | School of Radiation Medicine and Protection, Soochow University Medical College, Suzhou 215123, China | | | SHEN Lin-ling | School of Radiation Medicine and Protection, Soochow University Medical College, Suzhou 215123, China | | | ZHAO Lin | School of Radiation Medicine and Protection, Soochow University Medical College, Suzhou 215123, China | | | JIAO Yang | School of Radiation Medicine and Protection, Soochow University Medical College, Suzhou 215123, China | | | XU Jia-ying | School of Radiation Medicine and Protection, Soochow University Medical College, Suzhou 215123, China | | | FAN Sai-jun | School of Radiation Medicine and Protection, Soochow University Medical College, Suzhou 215123, China | sjfan@suda.edu.cn |
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| Abstract:: |
| Objective To explore the influence of honokiol on growth, cell cycle and radiosensitivity in nasopharyngeal carcinoma cells, CNE-1 and CNE-2. Methods MTT assay and clonogenic assay were used to detect cell growth and survival respectively. Flow cytometry was employed to analyze cell cycle progression. Annexin V-FITC kit was used to detect cell apoptosis. Western blot assay was applied to examine protein expression. Results Honokiol signficantly inhibited proliferation of CNE-1 and CNE-2 cells in a dose and time dependent manner, the IC50 value was 2.84 and 2.68 μmol/L(24 h) and 2.50 and 2.20 μmol/L (48 h), respectively. After being treated with 2.5 μmol/L honokiol for 24 h,the ratios of early apoptosis, late apoptosis and necrosis were 24.53%, 23.05% and 7.13% in CNE-1 cells compared with the control group(t=-41.17, -8.18, -6.08, P<0.05). The expression levels of pro-apoptotic proteins Caspase-3 and Bax were significantly increased to 2.31 and 1.89 times(t=-15.92, -17.15, P<0.05), 4.43 and 1.85 times(t=-29.39, -13.47, P<0.05). Simultaneously the expression level of anti-apoptotic protein Bcl-2 was reduced by 2.22 and 2.74 times(t=26.94, 66.14, P<0.05) as compared with controls after being treated with 4 and 3 μmol/L honokiol. Additionally, honokiol at lower doses signicantly enhanced the senstivity of CNE-1 and CNE-2 cells to X-ray irradiation. The SER was 1.41 and 1.88 in CNE-1 and CNE-2 cells. 3 Gy irradiation of X-rays increased the proportion at G2/M state in both cell lines(t=-14.96,-19.26,P<0.05). Honokiol reduced the G2/M cell cycle arrest induced by irradiation significantly(t=7.65, 4.98, P<0.05). Simultaneously, cyclin B1 protein expression obviously elevated(t=-33.07, -73.49, P<0.05). Conclusions Honokiol is a potent inhibitor of nasopharyngeal carcinoma cell growth by inducing cell apoptosis and necrosis and works as a radiosensitizer by disrupting G2/M cell cycle checkpoint. |
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