| ZHANG Hui-fang,YANG Biao,DANG Xu-hong,LIU Jian-gong,XING Li-hong,GUO Yue-feng,ZUO Ya-hui,DUAN Zhi-kai,LIU Zhan-qi.Effects of accumulated 60Co γ-ray irradiation on the small molecular metabolites in rats urine[J].Chinese Journal of Radiological Medicine and Protection,2012,32(4):358-362 |
| Effects of accumulated 60Co γ-ray irradiation on the small molecular metabolites in rats urine |
| Received:March 15, 2012 |
| DOI：10.3760/cma.j.issn.0254-5098.2012.04.006 |
| KeyWords:Accumulated irradiation Urinary metabolites Nuclear resonance spectroscopy Principal component analysis |
| FundProject: |
| Author Name | Affiliation | E-mail | | ZHANG Hui-fang | China Institute for Radiation Protection, Taiyuan 030006, China | | | YANG Biao | China Institute for Radiation Protection, Taiyuan 030006, China | | | DANG Xu-hong | China Institute for Radiation Protection, Taiyuan 030006, China | | | LIU Jian-gong | China Institute for Radiation Protection, Taiyuan 030006, China | | | XING Li-hong | China Institute for Radiation Protection, Taiyuan 030006, China | | | GUO Yue-feng | China Institute for Radiation Protection, Taiyuan 030006, China | microjenney@163.com | | ZUO Ya-hui | China Institute for Radiation Protection, Taiyuan 030006, China | | | DUAN Zhi-kai | China Institute for Radiation Protection, Taiyuan 030006, China | | | LIU Zhan-qi | China Institute for Radiation Protection, Taiyuan 030006, China | |
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| Abstract:: |
| Objective To explore the effects of accumulated 60Co γ-ray irradiation on small molecular metabolites in rats urine.Methods Ten healthy male SD rats were irradiated by 60Co γ-rays in 5 days and the accumulated doses were 0, 0.2, 0.4, 0.6, 0.8, 1.0 Gy, respectively. The metabolites in urine of different groups were measured with 1H-NMR combined with principal components analysis (PCA) and partial least square discriminate analysis (PLS-DA). Results The metabolites in rat urine were obviously changed after irradiation. Compared with control group, the amount of acetoacetate decreased after irradiation(t=29.7-30.7,P<0.05), but its relative level was stable when the dose increased (P>0.05). Meanwhile, the relative level of hippuric acid increased(t=4.4-21.6,P<0.05)especially when the accumulated dose was higher than 1 Gy (t= 21.6,P<0.05). The relative level of proline, taurine and trimethylamine-N-oxide increased after irradiation with the same trend(t=3.5-13.4,4.7-11.5,2.9-12.7,P<0.05). Conclusions The acetoacetate, hippuric acid, proline, taurine, and trimethylamine-N-oxide may be applicable for biomarkers of accumulative irradiation on rat. |
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