| WANG Qin,WANG Ying-ai,SONG Li,ZHANG Heng,LIU Qiang,FU Yue,CHEN Feng-hua,FAN Fei-yue,LI Jin.Synergic effect of human IL-21 gene transfer combined with γ-ray irradiation on the growth of cervical carcinoma HeLa cells[J].Chinese Journal of Radiological Medicine and Protection,2012,32(4):350-353 |
| Synergic effect of human IL-21 gene transfer combined with γ-ray irradiation on the growth of cervical carcinoma HeLa cells |
| Received:December 15, 2011 |
| DOI：10.3760/cma.j.issn.0254-5098.2012.04.004 |
| KeyWords:Interleukin-21 gene Irradiation Cervical carcinoma cell Growth inhibition |
| FundProject:天津市自然科学基金(09JCYBJC09300) |
| Author Name | Affiliation | E-mail | | WANG Qin | Peking Union Medical College, Institute of Radiation Medicine, Chinese Academy of Medical Sciences, Tianjin Key Laboratory of Molecular Nuclear Medicine, Tianjin 300192, China | | | WANG Ying-ai | 东莞市樟木头人民医院 | | | SONG Li | Peking Union Medical College, Institute of Radiation Medicine, Chinese Academy of Medical Sciences, Tianjin Key Laboratory of Molecular Nuclear Medicine, Tianjin 300192, China | | | ZHANG Heng | Peking Union Medical College, Institute of Radiation Medicine, Chinese Academy of Medical Sciences, Tianjin Key Laboratory of Molecular Nuclear Medicine, Tianjin 300192, China | | | LIU Qiang | Peking Union Medical College, Institute of Radiation Medicine, Chinese Academy of Medical Sciences, Tianjin Key Laboratory of Molecular Nuclear Medicine, Tianjin 300192, China | | | FU Yue | Peking Union Medical College, Institute of Radiation Medicine, Chinese Academy of Medical Sciences, Tianjin Key Laboratory of Molecular Nuclear Medicine, Tianjin 300192, China | | | CHEN Feng-hua | Peking Union Medical College, Institute of Radiation Medicine, Chinese Academy of Medical Sciences, Tianjin Key Laboratory of Molecular Nuclear Medicine, Tianjin 300192, China | | | FAN Fei-yue | Peking Union Medical College, Institute of Radiation Medicine, Chinese Academy of Medical Sciences, Tianjin Key Laboratory of Molecular Nuclear Medicine, Tianjin 300192, China | faithyfan@yahoo.con | | LI Jin | Peking Union Medical College, Institute of Radiation Medicine, Chinese Academy of Medical Sciences, Tianjin Key Laboratory of Molecular Nuclear Medicine, Tianjin 300192, China | |
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| Abstract:: |
| Objective To study the combined effect of interleukin-21 gene transfer and ionizing radiation on the growth of cervical carcinoma HeLa cells.Methods Previously constructed Ad-IL-21 gene was amplified by infecting 293A cells and the titer was measured by TCID50 method. HeLa cells were transfected with Ad-IL-21 and then irradiated with 6 Gy 137Cs γ-rays. The cells were divided into 5 groups,including blank control,Ad-LacZ group,Ad-IL-21 group,radiation group and Ad-IL-21 combined with radiation group (combination group). The cell growth, cell cycle, apoptosis, and the expressions of IL-21 gene and protein in HeLa cells were detected. Results Ad-IL-21 was successfully amplified and the titer of Ad-IL-21 was 9×1010 pfu/ml. Compared with Ad-IL-21 group and radiation group,the cell growth of combination group was significantly inhibited at 96 h after transfection (F=85.26,72.98,P<0.05). The cells in combination group were arrested in G1 phase and decreased at S phase(F=36.69,34.83, P<0.05), while the cellular apoptosis increased markedly(F=28.23,25.57, P<0.05). The gene expression of IL-21 in the combination group was 1.54- and 2.43-fold of Ad-IL-21 group and blank control group, respectively(F=22.31,36.65, P<0.05), while the protein expression of IL-21 in the combination group was 1.62-fold and 2.31-fold of Ad-IL-21 group and blank control group, respectively(F=27.36,35.86, P<0.05). Conclusions Ad-IL-21 gene transfection combined with radiation has synergic effect on the inhibition of cervical carcinoma cell growth. |
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