| QIAO Qiao,LI Guang.Ionizing radiation activates the HIF-1α-Survivin pathway in human lymphoma cells[J].Chinese Journal of Radiological Medicine and Protection,2012,32(3):274-277 |
| Ionizing radiation activates the HIF-1α-Survivin pathway in human lymphoma cells |
| Received:November 11, 2011 |
| DOI：10.3760/cma.j.issn.0254-5098.2012.03.013 |
| KeyWords:Lymphoma Hypoxia inducible factor-1α Survivin Ionizing radiation |
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| Abstract:: |
| Objective To determine the levels of radiation-induced expressions of HIF-1α and Survivin in three human lymphoma cell lines with or without HIF-1α specific antagonist or HIF-1α siRNA transfection and to explore the possible mechanism of cell radioresistance.Methods Namalwa,Ramos,Raji cells were irradiated with X-rays with or without the combination of echinomycin (EC), a HIF-1α specific inhibitor, or transfection of HIF-1α siRNA. Then the expression levels of HIF-1α and Survivin proteins and mRNAs were determined by Western blot and real-time PCR, respectively.Results After irradiation, the expression level of HIF-1α protein was up-regulated at 10-20 h followed by up-regulation of survivin protein at 24 h (t=7.53-31.31,P<0.01) in three lymphoma cell lines. Radiation-induced up-expression of survivin protein was significantly reduced by either HIF-1α inhibition or EC treatment (t=7.21-32.81,P<0.01). However, radiation failed to induce up-expression of survivin at both mRNA and protein levels after transfection with HIF-1α siRNA.Conclusions HIF-1α-Survivin pathway is activated in human lymphoma cells after radiation, which might be involved in the cell radioresistance. |
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