| LIU Xiao-qun,QIAO Tian-kui,CHEN Wei,et al.Role of ATM kinase in the effect of CpG ODN7909 on X-ray-induced G2/M phase arrest and apoptosis in A549 cells[J].Chinese Journal of Radiological Medicine and Protection,2012,32(3):270-273 |
| Role of ATM kinase in the effect of CpG ODN7909 on X-ray-induced G2/M phase arrest and apoptosis in A549 cells |
| Received:November 20, 2011 |
| DOI：10.3760/cma.j.issn.0254-5098.2012.03.012 |
| KeyWords:Unmethylated cytosine-phosphate-guanine oligodeoxynucleotides X-rays Ataxiatelangiectasia mutated kinase Cell cycle Apoptosis |
| FundProject: |
| Author Name | Affiliation | E-mail | | LIU Xiao-qun | Department of Oncology, Affiliated Jinshan Hospital, Fudan University, Shanghai 200540, China | | | QIAO Tian-kui | Department of Oncology, Affiliated Jinshan Hospital, Fudan University, Shanghai 200540, China | qiaotk@yahoo.com.cn | | CHEN Wei | Department of Oncology, Affiliated Jinshan Hospital, Fudan University, Shanghai 200540, China | | | YUAN Su-juan | Department of Oncology, Affiliated Jinshan Hospital, Fudan University, Shanghai 200540, China | |
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| Abstract:: |
| Objective To investigate the relationship between the effect of unmethylated cytosine-phosphate-guanine oligodeoxynucleotide(CpG ODN)7909 on X-ray-induced G2/M phase arrest and apoptosis and the phosphorylation of ATM kinase.Methods Human lung adenocarcinoma A549 cells were randomly classified into five groups, control, CpG group, irradiation group, CpG + irradiation group and ATM siRNA + CpG + irradiation group. Cell survival fraction was evaluated by clonogenic assay. Cell cycle and apoptosis were analyzed by flow cytometry. The expressions of ATM, checkpoint kinase-2 (Chk2) and p53 were detected with Western blot.Results Compared to the situation under X-ray irradiation alone, decreased cell clonogenic survival, prolonged G2/M arrest, and increased cell apoptosis were observed after the combination treatment with CpG ODN7909 and X-rays (t=13.41,17.32 and 7.71,P<0.05). Moreover, the phosphorylations on ATM, Chk2, and p53 were increased in the irradiated A549 cells that had been pre-treated with CpG ODN7909. After ATM siRNA interfering, abrogation of G2/M arrest, reduction of apoptosis, and decrease of Chk2 and p53 phosphorylation were found in A549 cells treated with CpG ODN7909 and X-rays (t=26.84,2.98,47.24 and 67.47,P<0.05). Conclusions CpG ODN7909 can enhance the X-ray-induced phosphorylation of ATM kinase in human lung adenocarcinoma cells in vitro, which might be involved in regulating G2/M phase arrest and apoptosis. |
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