| SUN Ke-kang,ZHAO Lin,XU Jia-ying,et al.Effect of ionizing radiation and cisplatin on the expression of TOB1 in human lung cancer cell lines[J].Chinese Journal of Radiological Medicine and Protection,2012,32(3):263-265 |
| Effect of ionizing radiation and cisplatin on the expression of TOB1 in human lung cancer cell lines |
| Received:August 18, 2011 |
| DOI：10.3760/cma.j.issn.0254-5098.2012.03.010 |
| KeyWords:Lung cancer Ionizing radiation Cisplatin Transducer of erbB2.1 |
| FundProject:教育部博士点新教师基金(K512602110) |
| Author Name | Affiliation | E-mail | | SUN Ke-kang | School of Radiation Medicine and Protection, Medical College, Soochow University, Suzhou 215123, China | | | ZHAO Lin | School of Radiation Medicine and Protection, Medical College, Soochow University, Suzhou 215123, China | | | XU Jia-ying | School of Radiation Medicine and Protection, Medical College, Soochow University, Suzhou 215123, China | | | JIAO Yang | School of Radiation Medicine and Protection, Medical College, Soochow University, Suzhou 215123, China | jiaoyang@suda.edu.cn |
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| Abstract:: |
| Objective To explore the effect of ionizing radiation and cisplatin on the expression of Transducer of erbB2,1 (TOB1) in human lung adenocareinoma SPCA-1 and LTEP-α-2 cell lines.Methods SPCA-1 and/or LTEP-α-2 cells were respectively irradiated with 2,4,6,8,and 10 Gy X-rays generated by a linear accelerator (with the source skin distance of 100 cm and dose rate of 200 cGy/min). The cell molecular samples were subtracted at 6,12,18,and 24 h after 6 Gy irradiation. For X-rays and cisplatin combination treatment, cells were divided into radiation group (6 Gy X-rays), cisplatin group (20 mol/L cisplatin), and combination group (6 Gy X-rays+20 mol/L cisplatin). SPCA-1 and LTEP-α-2 cells without any treatment were used as control group. The relative levels of TOB1 mRNA and protein expression were detected by rigorously controlled semi-quantitative RT-PCR and Western blot analysis with quantitation of the mRNA/protein bands by densitometry.Results The expression level of TOB1 was significantly increased in both mRNA and protein level after radiation exposure (t=8.25-24.48, P<0.05). For time-dependent induction of TOB1, the expression was significantly increased 6 h after X-rays exposure (t=14.23-15.82, P<0.05). More significant increase of TOB1 expression was identified after the combination treatment of X-rays and cisplatin, compared to that of the radiation and/or cisplatin treatment alone (t=11.21-13.67, P<0.05).Conclusions Ionizing radiation and cisplatin could upregulate the expression of TOB1 in lung cancer cells in both mRNA and protein levels. TOB1 may be a molecular target for ionizing radiation and cisplatin treatment in lung cancer and it may have potential implication in evaluating the curative efficiency of chemo-and radio-therapy. |
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