| ZHU Xiao-dong,HUANG Shi-ting,QU Song,SU Fang,GUO Ya,WANG Jin-zi.Comparative proteomic analysis on radioresistant nasopharyngeal carcinoma cell[J].Chinese Journal of Radiological Medicine and Protection,2012,32(3):245-248 |
| Comparative proteomic analysis on radioresistant nasopharyngeal carcinoma cell |
| Received:May 10, 2011 |
| DOI：10.3760/cma.j.issn.0254-5098.2012.03.006 |
| KeyWords:Nasopharyngeal carcinoma Radioresistance Comparative proteomics |
| FundProject:国家自然科学基金(30860329);广西大型仪器协作共用网测试项目(851-2009-055);广西2010年研究生教育创新计划项目(2010105981002M215) |
| Author Name | Affiliation | E-mail | | ZHU Xiao-dong | Department of Radiotherapy, Cancer Hospital of Guangxi Medical University, Cancer Institute of Guangxi Zhuang Autonomous Region, Nanning 530021, China | zhuxiaodong1966@yahoo.com.cn | | HUANG Shi-ting | Department of Radiotherapy, Cancer Hospital of Guangxi Medical University, Cancer Institute of Guangxi Zhuang Autonomous Region, Nanning 530021, China | | | QU Song | Department of Radiotherapy, Cancer Hospital of Guangxi Medical University, Cancer Institute of Guangxi Zhuang Autonomous Region, Nanning 530021, China | | | SU Fang | Department of Radiotherapy, Cancer Hospital of Guangxi Medical University, Cancer Institute of Guangxi Zhuang Autonomous Region, Nanning 530021, China | | | GUO Ya | Department of Radiotherapy, Cancer Hospital of Guangxi Medical University, Cancer Institute of Guangxi Zhuang Autonomous Region, Nanning 530021, China | | | WANG Jin-zi | 广西大学生命科学与技术学院 | |
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| Abstract:: |
| Objective To discover radioresistance-associated proteins by performing comparative proteomic analysis on nasopharyngeal carcinoma cell lines.Methods The total proteins were extracted from radioresistant human nasopharyngeal carcinoma cell line CNE-2R and its parental cell line CNE-2, respectively. These proteins were separated by high quality two-dimensional polyacrylamide gel electrophoresis (2-DE) and then the 2-DE profiles were screened for differentially expressed protein spots by the Image Master 5.0 software. Those spots were identified by a matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry.Results 32 significantly differentially expressed protein spots were screened in two different radiosensitivity cell lines and 11 proteins were identified by tandem mass spectrometry, among which 3 proteins were up-regulated in radioresistant human nasopharyngeal carcinoma cell line CNE-2R and the other 8 proteins were down-regulated.Conclusions The differentially expressed proteins of nasopharyngeal carcinoma cells with different radiosensitivity were mainly involved in apoptosis regulation, DNA damage and repair, cell cycle regulation, RNA transcription, cell signaling, cytoskeleton formation and radiation stress responses. |
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