ZHAO Lin,SUN Ke-kang,SHEN Lin-ling,et al.Effect of epigallocatechin-3-gallate on radiosensitivity of CNE-1 cells[J].Chinese Journal of Radiological Medicine and Protection,2012,32(3):236-240
Effect of epigallocatechin-3-gallate on radiosensitivity of CNE-1 cells
Received:October 24, 2011  
DOI:10.3760/cma.j.issn.0254-5098.2012.03.004
KeyWords:EGCG  Nasopharyngeal carcinoma  Radiosensitivity  Cell cycle  Apoptosis
FundProject:国家科学自然研究基金面上项目(81071906;81172127);教育部"长江学者和创新团队发展计划资助"项目(IRT0849);江苏高校优势学科建设工程资助项目(PAPD)
Author NameAffiliationE-mail
ZHAO Lin School of Radiation Medicine and Protection, Soochow University Medical College, Suzhou 215123, China  
SUN Ke-kang School of Radiation Medicine and Protection, Soochow University Medical College, Suzhou 215123, China  
SHEN Lin-ling School of Radiation Medicine and Protection, Soochow University Medical College, Suzhou 215123, China  
JIAO Yang School of Radiation Medicine and Protection, Soochow University Medical College, Suzhou 215123, China  
XU Jia-ying School of Radiation Medicine and Protection, Soochow University Medical College, Suzhou 215123, China  
FAN Sai-jun School of Radiation Medicine and Protection, Soochow University Medical College, Suzhou 215123, China sjfan@suda.edu.cn 
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Abstract::
      Objective To investigate the effect of EGCG on the radiosensitivity of human nasopharyngeal carcinoma CNE-1 cells.Methods CNE-1 cells were divided into four groups: control, EGCG treatment, UVC or X-ray exposure, and EGCG combined with UVC or X-rays. After treatment with different concentrations of EGCG for 24, 48 and 72 h and UVC or X-rays, cell growth was determined with MTT assay, cell survival was measured with clonogenic assay, cell cycle was detected with flow cytometry, cell apoptosis was detected by the annexin V-FITC cell apoptosis kit, and protein expression was assayed by Western blot.Results EGCG inhibited cell growth in a dose-and time-dependent manner(r = 0.817 and 0.364). Compared with UVC or X-ray irradiation alone, the radiosensitivity of CNE-1 cells was enhanced by 2 h pre-treatment of 50 μmol/L EGCG,which disrupted S phase arrest caused by UVC(t =18.68,P<0.05)and increased the population of S and G2/M arrest caused by X-rays(t =7.11 and 6.99,P<0.05). UVC could cause a significant increase of sub-G1 population(t =6.67,P<0.05)and Annexin V-FITC assay indicated apoptosis was further elevated by EGCG(t =10.28,P<0.05). However, no significant induction of apoptosis was observed in the cells either irradiated with X-rays alone or combinationly treated with EGCG and X-rays. The combination treatment of EGCG and UVC significantly increased the expression of Bax and Caspase-3 proteins, but failed to affect Bcl-2 protein expression.Conclusions EGCG enhances the growth inhibition of CNE-1 cells caused by UVC or X-rays, which is relevant to apoptosis induction or cell cycle arrest.
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