| ZHAO Lin,SUN Ke-kang,SHEN Lin-ling,JIAO Yang,XU Jia-ying,FAN Sai-jun.Effect of epigallocatechin-3-gallate on radiosensitivity of CNE-1 cells[J].Chinese Journal of Radiological Medicine and Protection,2012,32(3):236-240 |
| Effect of epigallocatechin-3-gallate on radiosensitivity of CNE-1 cells |
| Received:October 24, 2011 |
| DOI：10.3760/cma.j.issn.0254-5098.2012.03.004 |
| KeyWords:EGCG Nasopharyngeal carcinoma Radiosensitivity Cell cycle Apoptosis |
| FundProject:国家科学自然研究基金面上项目(81071906;81172127);教育部"长江学者和创新团队发展计划资助"项目(IRT0849);江苏高校优势学科建设工程资助项目(PAPD) |
| Author Name | Affiliation | E-mail | | ZHAO Lin | School of Radiation Medicine and Protection, Soochow University Medical College, Suzhou 215123, China | | | SUN Ke-kang | School of Radiation Medicine and Protection, Soochow University Medical College, Suzhou 215123, China | | | SHEN Lin-ling | School of Radiation Medicine and Protection, Soochow University Medical College, Suzhou 215123, China | | | JIAO Yang | School of Radiation Medicine and Protection, Soochow University Medical College, Suzhou 215123, China | | | XU Jia-ying | School of Radiation Medicine and Protection, Soochow University Medical College, Suzhou 215123, China | | | FAN Sai-jun | School of Radiation Medicine and Protection, Soochow University Medical College, Suzhou 215123, China | sjfan@suda.edu.cn |
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| Abstract:: |
| Objective To investigate the effect of EGCG on the radiosensitivity of human nasopharyngeal carcinoma CNE-1 cells.Methods CNE-1 cells were divided into four groups: control, EGCG treatment, UVC or X-ray exposure, and EGCG combined with UVC or X-rays. After treatment with different concentrations of EGCG for 24, 48 and 72 h and UVC or X-rays, cell growth was determined with MTT assay, cell survival was measured with clonogenic assay, cell cycle was detected with flow cytometry, cell apoptosis was detected by the annexin V-FITC cell apoptosis kit, and protein expression was assayed by Western blot.Results EGCG inhibited cell growth in a dose-and time-dependent manner(r = 0.817 and 0.364). Compared with UVC or X-ray irradiation alone, the radiosensitivity of CNE-1 cells was enhanced by 2 h pre-treatment of 50 μmol/L EGCG,which disrupted S phase arrest caused by UVC(t =18.68,P<0.05)and increased the population of S and G2/M arrest caused by X-rays(t =7.11 and 6.99,P<0.05). UVC could cause a significant increase of sub-G1 population(t =6.67,P<0.05)and Annexin V-FITC assay indicated apoptosis was further elevated by EGCG(t =10.28,P<0.05). However, no significant induction of apoptosis was observed in the cells either irradiated with X-rays alone or combinationly treated with EGCG and X-rays. The combination treatment of EGCG and UVC significantly increased the expression of Bax and Caspase-3 proteins, but failed to affect Bcl-2 protein expression.Conclusions EGCG enhances the growth inhibition of CNE-1 cells caused by UVC or X-rays, which is relevant to apoptosis induction or cell cycle arrest. |
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