| SONG Chuan-jun,MENG Xing-jun,XIE Ling,et al.Effect of heme oxygenase-1 on radiation-induced skin injury[J].Chinese Journal of Radiological Medicine and Protection,2012,32(3):230-232 |
| Effect of heme oxygenase-1 on radiation-induced skin injury |
| Received:July 27, 2011 |
| DOI：10.3760/cma.j.issn.0254-5098.2012.03.002 |
| KeyWords:Heme oxygenase Adenovirus Irradiation-induced skin injury Gene therapy |
| FundProject:国家自然科学基金(81102078) |
| Author Name | Affiliation | E-mail | | SONG Chuan-jun | Department of Radiotherapy, Suzhou Hospital Affiliated to Nanjing Medical University, Suzhou 215001, China | | | MENG Xing-jun | 南京医科大学附属苏州医院肿瘤诊断与治疗重点实验室 | | | XIE Ling | Department of Radiotherapy, Suzhou Hospital Affiliated to Nanjing Medical University, Suzhou 215001, China | | | CHEN Qing | Department of Radiotherapy, Suzhou Hospital Affiliated to Nanjing Medical University, Suzhou 215001, China | | | ZHOU Jun-dong | Department of Radiotherapy, Suzhou Hospital Affiliated to Nanjing Medical University, Suzhou 215001, China | | | ZHANG Shu-yu | 苏州大学放射医学与防护学院 | | | WU Jin-chang | Department of Radiotherapy, Suzhou Hospital Affiliated to Nanjing Medical University, Suzhou 215001, China | wjinchang@sina.com |
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| Abstract:: |
| Objective To investigate the effect of heme oxygenase-1(HO-1) on the acute radiation-induced skin injury by gene transfer.Methods Thirty-three male SD rats were randomly divided into three groups as PBS-injected group, Ad-EGFP-injected group and Ad-HO-1-injected group (n=11). In each group, three rats were used for determining the expression of target gene and the other rats were irradiated on the buttock skin with 40 Gy electron beam generated by a linear accelerator. Immediately after irradiation, rats were administered with a subcutaneous injection of PBS, Ad-EGFP or Ad-HO-1, respectively. Subsequently, the skin reactions were measured twice a week using the semi-quantitative skin injury scale.Results The strong positive expression of HO-1 was observed in subcutaneous dermal tissue after injection of Ad-HO-1.Compared to the PBS-injected group or the Ad-EGFP-injected group, a significant mitigation of skin injury was observed in Ad-HO-1-injected mice 14 d after irradiation (q=0.000－0.030, P<0.05).Conclusions HO-1 could significantly mitigate radiation-induced acute skin injury and Ad-HO-1 could be used to treat radiation-induced skin injury. |
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