| YAN Peng,JIANG Qi-sheng,LI Feng-sheng,et al.Effects of low dose X-ray irradiation on antigen presentation and IL-12 secretion in human dendritic cells in vitro[J].Chinese Journal of Radiological Medicine and Protection,2012,32(3):225-229 |
| Effects of low dose X-ray irradiation on antigen presentation and IL-12 secretion in human dendritic cells in vitro |
| Received:December 21, 2011 |
| DOI：10.3760/cma.j.issn.0254-5098.2012.03.001 |
| KeyWords:X-rays Dendritic cells A549 cells Interleukin-12 |
| FundProject:国家自然科学基金(81172130) |
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| Abstract:: |
| Objective To explore the effects of low dose X-ray irradiation on the ability of antigen presentation and IL-12 secretion in human dendritic cells that had been cultured for different time in vitro. Methods The human peripheral blood mononuclear cells (PBMC) were collected and differentiated to dendritic cells (DCs) by rhGM-CSF and rhIL-4 treatment in vitro. The DCs were divided into 3 groups, group A: DCs were cultured for 2 d and then irradiated with 0.05, 0.1, 0.2 and 0.5 Gy X-rays; group B: DCs were cultured for 6 d and then irradiated as above; group C: DCs were cultured without irradiation. At 8 d of cell culture, the DCs were applied to activate T cells and CCK-8 was used to detect MLR (mixed lymphocyte reaction), and the antigen presentation ability of DCs was evaluated. MTT assay was also used to test the cell-killing effect of the activated T-cells on A549 cells. IL-12 in the culture medium of DCs was detected by ELISA.Results After irradiation with 0.2 and 0.5 Gy X-rays, the antigen presentation ability of DCs was decreased in group A (t=2.79 and 3.71, P<0.05), but significantly increased in group B (t=3.60 and 3.11, P<0.05). The ability of the T cell activation was detected and the proliferation of A549 cells was slightly inhibited by the DCs in group A (t=2.89 and 2.91, P<0.05), but was obviously inhibited by the DCs in group B (t=2.91 and 2.82, P<0.05). Meanwhile, the level of IL-12 was dramatically decreased in group A (t=4.44 and 6.93, P<0.05) , but was increased in group B (t=3.51 and 4.12,P<0.05).Conclusions The abilities of antigen presentation and proliferation inhibition of DCs could be down-regulated by low dose(<0.5 Gy) of X-ray irradiation at the early stage of DCs, but was up-regulated at the late stage of DCs culture. |
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