| QIAO Qiao,JIANG Yuan-jun,LI Guang.Inhibition of NF-κB enhanced X-ray irradiation induced apoptosis in human non-Hodgkin lymphoma[J].Chinese Journal of Radiological Medicine and Protection,2012,32(2):182-185 |
| Inhibition of NF-κB enhanced X-ray irradiation induced apoptosis in human non-Hodgkin lymphoma |
| Received:July 17, 2011 |
| DOI：10.3760/cma.j.issn.0254-5098.2012.02.019 |
| KeyWords:Lymphoma|NF-κB|X-rays|Survivin|Apoptosis |
| FundProject:辽宁省教育厅基金(2009A754) |
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| Abstract:: |
| Objective To investigate the role of NF-κB in radiation-induced apoptosis of human non-Hodgkin lymphoma (NHL) cells and the mechanism involved. Methods Three human non-Hodgkin lymphoma cell lines, Namalwa,Ramos, and Raji cells were divided into control, IR and IR+QNZ (10 nmol/L) groups,respectively. Annexin-Ⅴ kit was used to determine cell apoptosis. Protein expression levels of Survivin, Bax, Bcl-2 and cleaved Caspase-3 were evaluated by Western blot. Survivin mRNA was quantified by real-time PCR. Results Inhibition of NF-κB by QNZ pretreatment significantly enhanced X-ray induced apoptosis in human NHL cells in a dose-dependent manner (t=2.93-12.52, P<0.05). At the same time, QNZ significantly reversed the expression levels of Survivin protein and mRNA that were up-regulated by radiation(t=3.29-16.72,P<0.05). QNZ also increased the levels of Caspase-3 and pro-apoptotic protein Bax, but reduced anti-apoptotic protein Bcl-2 expression level and hence the ratio of Bcl-2/Bax. Conclusions Inhibition of NF-κB could enhance radiation-induced cell apoptosis in human NHL cells through down-regulating Survivin expression and decreasing Bcl-2/Bax ratio. |
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