| ZHAO Lin,WANG Yan-su,REN Hang,et al.Ethanol decreases radiosensitivity of human breast cancer MCF-7 cells[J].Chinese Journal of Radiological Medicine and Protection,2012,32(2):162-165 |
| Ethanol decreases radiosensitivity of human breast cancer MCF-7 cells |
| Received:November 15, 2011 |
| DOI：10.3760/cma.j.issn.0254-5098.2012.02.014 |
| KeyWords:Ethanol|Breast cancer|Radiosensitivity|Cell cycle|Apoptosis |
| FundProject:国家自然科学基金(81071906;81172127);长江学者和创新团队发展计划资助项目(IRT0849);江苏省省属高校自然科学重大基础研究项目(SZ126933);江苏省高等学校优秀科技创新团队项目(SZ132901);江苏省高校优势学科建设工程资助项目(PAPD) |
| Author Name | Affiliation | E-mail | | ZHAO Lin | School of Radiation Medicine and Public Health, Soochow University Medical College, Suzhou 215123, China | | | WANG Yan-su | School of Radiation Medicine and Public Health, Soochow University Medical College, Suzhou 215123, China | | | REN Hang | School of Radiation Medicine and Public Health, Soochow University Medical College, Suzhou 215123, China | | | XU Jia-ying | School of Radiation Medicine and Public Health, Soochow University Medical College, Suzhou 215123, China | | | JIAO Yang | School of Radiation Medicine and Public Health, Soochow University Medical College, Suzhou 215123, China | | | FAN Sai-jun | School of Radiation Medicine and Public Health, Soochow University Medical College, Suzhou 215123, China | sjfan@suda.edu.cn |
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| Abstract:: |
| Objective To investigate the effect of ethanol on radiosensitivity of human breast cancer MCF-7 cells. Methods Human breast cancer MCF-7 cells were divided into four groups including control group, ethanol treatment group, X-ray exposed group, and ethanol combined with X-ray group. Clonogenic assay was used to determine cell survival. Flow cytometry was employed to analyze cell cycle progression. Annexin V-FITC kit was used to determine cell apoptosis induction. Results Ethanol(50 and 100 mmol/L,50 h)had no influence on MCF-7 cell growth(t=0.82,1.15,P>0.05). The radiosensitivity of MCF-7 cells was reduced when the cells were pretreated with 50 mmol/L ethanol (t=4.15,P<0.05) and 100 mmol/L ethanol(t=10.28,P<0.05)for 2 h. Compared with irradiation with X-ray alone, ethanol treatment decreased G2/M phase arrest(t=7.18,P<0.05) and sub-G1 population(an indicator of apoptosis induction)(t=5.39,P<0.05). A decrease of advanced and early apoptosis in the cells pretreated with ethanol was also confirmed by Annexin V-FITC apoptosis assay(t=4.86,7.59,P<0.05). Conclusions Ethanol causes radioresistance in human breast cancer MCF-7 cells, where the decreases of radiation-induced G2/M phase arrest and apoptosis may be involved. |
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