| GOU Qiao,WANG Chun-yan,ZHANG Cui-lan,et al.Study on oxidative lipid and DNA damages in the malignant transformed BEP2D cells induced by α-particle exposure[J].Chinese Journal of Radiological Medicine and Protection,2012,32(2):157-161 |
| Study on oxidative lipid and DNA damages in the malignant transformed BEP2D cells induced by α-particle exposure |
| Received:August 03, 2011 |
| DOI：10.3760/cma.j.issn.0254-5098.2012.02.013 |
| KeyWords:Human bronchial epithelial cell line|α-particle|Reactive oxygen radicals|Oxidative damages|Carcinogenesis |
| FundProject:国家自然科学基金(81000862);中国科学技术部社会公益研究专项(2005DIB1J087);卫生行业科研专项(200802018) |
| Author Name | Affiliation | E-mail | | GOU Qiao | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | WANG Chun-yan | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | ZHANG Cui-lan | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | TONG Peng | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | SU Xu | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | suxu@nirp.cn |
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| Abstract:: |
| Objective To investigate the mechanism of malignant transformation in human bronchial epithelial cell line BEP2D exposed to α-particles. Methods The levels of intracellular ROS and malonaldehyde (MDA) in BEP2D, RH22 (passage 22 of α-particle-irradiated BEP2D cells) and BERP35T-1 cells (derived from nude mice bearing malignant transformed cells generated from the passage 35 of α-particle-irradiated BEP2D cells) were assayed with DCFH-DA and MDA kit, respectively. The expressions of 8-OH-dG and γ-H2AX in BEP2D, RH23 (passage 23 of α-particle-irradiated BEP2D cells) and BERP35T-1 cells were also measured with immunocytochemistry and immunofluorescence staining. Results Compared to BEP2D cells, the levels of ROS (t=4.30 and 3.94, P<0.05) and MDA (t=4.89 and 15.10, P<0.05) increased in RH22 and BERP35T-1 cells. The expressions of 8-OH-dG (t= 3.80 and 2.92, P<0.05) and γ-H2AX (t= 7.61 and 12.67, P<0.05) in RH23 and BERP35T-1 cells were also higher than those in BEP2D cells. Conclusions Oxidative stress induces lipid peroxidation and DNA damage leading to genomic instability, which could contribute to cellular malignant transforming process in the human bronchial epithelial cell line BEP2D with α-particle exposure. |
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