| WU Hui,WANG De-wen,WANG Shui-ming,et al.Effect of electromagnetic radiation at different bands on the expressions of SOX9 and WT1 in rat Sertoli cells[J].Chinese Journal of Radiological Medicine and Protection,2012,32(1):47-51 |
| Effect of electromagnetic radiation at different bands on the expressions of SOX9 and WT1 in rat Sertoli cells |
| Received:May 04, 2011 |
| DOI：10.3760/cma.j.issn.0254-5098.2012.01.011 |
| KeyWords:Electromagnetic radiation Sertoli cells SOX9 WT1 |
| FundProject:中国博士后科学基金资助项目(20090451503);军队"十一五"科技重点攻关项目(08G145) |
| Author Name | Affiliation | E-mail | | WU Hui | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | WANG De-wen | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | wangdewen1938@yahoo.com.cn | | WANG Shui-ming | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | ZUO Hong-yan | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | LI Yang | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | GAO Ya-bing | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | XU Xin-ping | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | ZHOU Hong-mei | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | SU Zhen-tao | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | YAO Hua | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | LI Ning | 解放军第三○九医院病理科 | | | PENG Rui-yun | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | |
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| Abstract:: |
| Objective To explore the changes of SOX9 and WT1 expressions in rat Sertoli cells irradiated by EMP (electromagnetic pulse), S-HPM (S-band high power microwave) and X-HPM (X-band high power microwave). Methods Primary Sertoli cells were isolated from 3-week-old Wistar rats and its purity was immunocytochemistrically indentified with WT1. After exposure to 6×104 V/m EMP, 100 mW/cm2 S-HPM and X-HPM for 4 min respectively, SOX9 and WT1 expressions in Sertoli cells were determined with real-time PCR and Western blot, respectively. Results SOX9 mRNA expression was decreased at 6 and 12 h post-irradiation of three different bands of electromagnetic microwave (F=15.20 and 4.84, P<0.05). SOX9 protein expression was also decreased at 6 and 24 h after irradiation (F=8.46 and 7.47, P<0.05). WT1 mRNA expression was decreased at 6 and 12 h (F=13.46 and 5.08, P<0.05), but its protein expression was decreased only at 24 h post-irradiation (F=10.26, P<0.05). Conclusions Three bands of electromagnetic radiation reduce the expressions of SOX9 and WT1 in rat Sertoli cells, which may provide molecular foundation for genital system hazards induced by microwave radiation. |
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