| DU Li-qing,BAI Jian-qiang,LIU Qiang,WANG Yan,ZHAO Peng,CHEN Feng-hua,WANG Hong,FAN Fei-yue.Correlation of RAD51 and radiosensitization of methotrexate[J].Chinese Journal of Radiological Medicine and Protection,2012,32(1):44-46 |
| Correlation of RAD51 and radiosensitization of methotrexate |
| Received:April 12, 2011 |
| DOI：10.3760/cma.j.issn.0254-5098.2012.01.010 |
| KeyWords:RAD51 Methotrexate Radiosensitization |
| FundProject: |
| Author Name | Affiliation | E-mail | | DU Li-qing | Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300192, China | | | BAI Jian-qiang | 天津骨科医院脊柱外科 | | | LIU Qiang | Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300192, China | | | WANG Yan | Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300192, China | | | ZHAO Peng | 华北煤炭医学院 | | | CHEN Feng-hua | Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300192, China | | | WANG Hong | Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300192, China | | | FAN Fei-yue | Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300192, China | faithyfan@yahoo.cn |
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| Abstract:: |
| Objective To evaluate the correlation between homologous recombination repair protein RAD51 and methotrexate-enhanced radiosensitivity. Methods Western blot and RT-PCR assays were used to detect RAD51 expression in HOS osteosarcoma cells exposed to γ-ray irradiation alone and in combination with methotrexate. Colony formation assay was used to test the survival fraction of HOS cells exposed to γ-rays and methotrexate. Results Methotrexate inhibited both protein and RNA expressions of RAD51, and the combination of radiation and methotrexate enhanced the inhibition of RAD51 expression. Moreover, transfection of cells with RAD51 gene decreased cellular sensitivity to methotrexate and γ-rays. The sensitizer enhancerment ratios after irradiation in combination with methotrexate were 1.51 and 0.99, respectively. Methotrenate was a preferred radiosensitizer to HOS cell. Conclusions RAD51 might be involved in the methotrexate-enhanced radiosensitivity. |
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