| LI Wen-yan,LIU Wei,MENG Xiao-jie.Simultaneous silence of bcl-2 and XIAP increases radiosensitivity of UMSCC12 head and neck cancer cells[J].Chinese Journal of Radiological Medicine and Protection,2012,32(1):40-43,59 |
| Simultaneous silence of bcl-2 and XIAP increases radiosensitivity of UMSCC12 head and neck cancer cells |
| Received:July 09, 2011 |
| DOI：10.3760/cma.j.issn.0254-5098.2012.01.009 |
| KeyWords:bcl-2 XIAP UMSCC12 Radiosensitization Apoptosis |
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| Abstract:: |
| Objective To investigate the radiosensitization effect of the simultaneous silence of bcl-2 and XIAP on human head and neck cancer cell line in vitro. Methods Four groups of UMSCC12 cells were transfected with siRNA-bcl-2, siRNA-XIAP, siRNA-bcl-2 plus siRNA XIAP, and siRNA control, respectively. The silence efficiency was tested by Western blot assay. Apoptosis was evaluated with the activities of caspase-3 and caspase-9, and radiosensitization effect was evaluated with clonogenic assay. Results Bcl-2 and XIAP protein expressions were effectively eliminated in the cells simultaneously transfected with siRNA-bcl-2 and siRNA-XIAP. Transfection of cells with bcl-2 siRNA increased radiation-induced apoptosis (t=5.32,6.27;P<0.05), but transfection with XIAP siRNA did not impact cell apoptosis. Since the simultaneous transfection of the above two siRNAs, the activities of caspase-3 and caspase-9 were increased by 1.36 and 1.34 times (t=11.47,6.22;P<0.05)in nonirradiated cells and increased by 1.72 and 1.98 times (t=12.02,20.14;P<0.05) in irradiated cells, respectively. The colonic assay showed that the SERs were 1.06, 1.15 and 1.41 for the cells transfected with XIAP siRNA, bcl-2 siRNA and both siRNAs, respectively. Conclusions Compared to single silence of XIAP or bcl-2, simultaneous silence of XIAP and bcl-2 offers a potential approach to improving the radiosensitivity of the head and neck cancer cells, and apoptosis might contribute to the enhancement of radiosensitization. |
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