| QIN Hong-ran,TIAN Mei,GAO Gang,RUAN Jian-lei,LIU Jian-xiang.Expressions of lung cancer related genes and miRNA in peripheral blood of the residents surrounding hot springs with extremely high radon[J].Chinese Journal of Radiological Medicine and Protection,2012,32(1):31-34 |
| Expressions of lung cancer related genes and miRNA in peripheral blood of the residents surrounding hot springs with extremely high radon |
| Received:March 23, 2011 |
| DOI：10.3760/cma.j.issn.0254-5098.2012.01.007 |
| KeyWords:Hot springs with high radon p53 miR-34a/b let-7a k-ras |
| FundProject:国家自然科学基金(31100606);中国疾病预防控制中心青年科研基金(2009A201) |
| Author Name | Affiliation | E-mail | | QIN Hong-ran | China CDC Key Laboratory of Radiological Protection and Nuclear Emergency, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | TIAN Mei | China CDC Key Laboratory of Radiological Protection and Nuclear Emergency, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | GAO Gang | China CDC Key Laboratory of Radiological Protection and Nuclear Emergency, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | RUAN Jian-lei | China CDC Key Laboratory of Radiological Protection and Nuclear Emergency, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | LIU Jian-xiang | China CDC Key Laboratory of Radiological Protection and Nuclear Emergency, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | jxliu@nirp.cn |
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| Abstract:: |
| Objective To investigate the expressions of lung cancer related genes and miRNA in peripheral blood of the residents surrounding the extremely high radon hot springs in Ruoergai County, Sichuan Province. Methods Peripheral blood samples were collected from the local residents. Expressions of lung cancer related genes (p53, k-ras) and miRNA (let-7a, miR-34a/b) were detected by real-time PCR and the protein expressions of p53 and k-ras were detected by Western blot. Results The expressions of p53 and k-ras mRNA of the residents in high radon area were 0.97 and 1.33 times of the control respectively (t=0.13,-1.12,P>0.05), and the p53 and k-ras protein levels were 0.70 and 1.23 times of the control respectively (t=0.72,0.46,P>0.05). The let-7a of the residents in high radon area was lower (t=1.63,P>0.05) while the miR-34a and miR-34b were significantly higher than those of the controls (t=-3.20,-3.32,P<0.05). Conclusions Based on the expressions of p53 and k-ras gene and miRNA, it can be concluded that the residents surrounding the high radon hot springs received radiation damage. |
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