| CUI Wei,YIN Ling-ling,DONG Ling-yue,LI Li,LIU Zhi-cheng,LI Yan-ling,AN Wei,LIU Xiao-dan,YANG Chuan-jie,ZHOU Ping-kun,DING Ku-ke.Study on the binding sites of radiosensitivity associated transcription factor in the promoter region of Ier5 gene[J].Chinese Journal of Radiological Medicine and Protection,2012,32(1):15-19 |
| Study on the binding sites of radiosensitivity associated transcription factor in the promoter region of Ier5 gene |
| Received:January 12, 2011 |
| DOI：10.3760/cma.j.issn.0254-5098.2012.01.004 |
| KeyWords:Immediate early response gene 5 Radiation sensitivity Transcription |
| FundProject:国家高科技研究发展计划(863) (2004AA221160);国家自然科学基金(30770533;31170806);北京市自然科学基金(7092013);北京市教育委员会科技发展基金(Km200710025007) |
| Author Name | Affiliation | E-mail | | CUI Wei | Department of Biomechanics and Rehabilitation Project, Captial Medical University, Beijing 100069, China | | | YIN Ling-ling | 河北医科大学第二附属医院消化内科 | | | DONG Ling-yue | 100069 北京, 首都医科大学生物医学细胞生物学系 | | | LI Li | 北京佑安医院性病艾滋病实验室 | | | LIU Zhi-cheng | Department of Biomechanics and Rehabilitation Project, Captial Medical University, Beijing 100069, China | | | LI Yan-ling | Department of Biomechanics and Rehabilitation Project, Captial Medical University, Beijing 100069, China | | | AN Wei | 100069 北京, 首都医科大学生物医学细胞生物学系 | | | LIU Xiao-dan | 军事医学科学院放射与辐射医学研究所 | | | YANG Chuan-jie | 河北医科大学第二附属医院消化内科 | | | ZHOU Ping-kun | 军事医学科学院放射与辐射医学研究所 | | | DING Ku-ke | Department of Biomechanics and Rehabilitation Project, Captial Medical University, Beijing 100069, China | shouding@ccmu.edu.cn |
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| Abstract:: |
| Objective To clarify the mechanism of immediate early response gene 5 (Ier5) transcription induced by radiation. Methods Deletant construction, site-specific mutagenesis, electrophoretic mobility shift assay(EMSA)and chromatin immunoprecipitation (ChIP) were used to forecast the promoter region, binding sites and transcription factors of Ier5 gene in HeLa cells. Results The promoter region of Ier5 gene might be in the region of Ier5-8 deletant (-408--238 bp). The Ier5 gene had two transcription factors of GCF and NFI, and GCF had two binding sites located in the region of -388--382 bp and -274--270 bp of Ier5 promoter. The binding site of NFI was located in -362--357 bp of Ier5 promoter. GCF could inhibit the expression of Ier5 gene and this inhibition was diminished when the radiation dose increased. In contrast, NFI increased the expression of Ier5. Conclusions The most possible region of Ier5 promoter is from -408 to -238 bp which has two binding sites for the radiosensitivity transcription factors of GCF and NFI that could negatively and positively regulate the expression of Ier5 respectively. |
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