| SONG Zhi-heng,LIU Xiao-dong,XU Hui-ying,LIANG Bing,JIA Li-li,KONG De-juan,YI He-qing,HE Meng-zi,MA Shu-mei.Changes of DRAM expression in radiation-induced autophagy in breast cancer cells[J].Chinese Journal of Radiological Medicine and Protection,2012,32(1):11-14 |
| Changes of DRAM expression in radiation-induced autophagy in breast cancer cells |
| Received:April 26, 2011 |
| DOI：10.3760/cma.j.issn.0254-5098.2012.01.003 |
| KeyWords:Autophagy X-rays DRAM gene |
| FundProject:国家自然科学基金(30970682;30770649) |
| Author Name | Affiliation | E-mail | | SONG Zhi-heng | Key Laboratory of Radiobiology, Ministry of Health, School of Public Health, Jilin University, Changchun 130021, China | | | LIU Xiao-dong | Key Laboratory of Radiobiology, Ministry of Health, School of Public Health, Jilin University, Changchun 130021, China | | | XU Hui-ying | Key Laboratory of Radiobiology, Ministry of Health, School of Public Health, Jilin University, Changchun 130021, China | | | LIANG Bing | Key Laboratory of Radiobiology, Ministry of Health, School of Public Health, Jilin University, Changchun 130021, China | | | JIA Li-li | Key Laboratory of Radiobiology, Ministry of Health, School of Public Health, Jilin University, Changchun 130021, China | | | KONG De-juan | Key Laboratory of Radiobiology, Ministry of Health, School of Public Health, Jilin University, Changchun 130021, China | | | YI He-qing | Key Laboratory of Radiobiology, Ministry of Health, School of Public Health, Jilin University, Changchun 130021, China | | | HE Meng-zi | Key Laboratory of Radiobiology, Ministry of Health, School of Public Health, Jilin University, Changchun 130021, China | | | MA Shu-mei | Key Laboratory of Radiobiology, Ministry of Health, School of Public Health, Jilin University, Changchun 130021, China | shumm2001@yahoo.com |
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| Abstract:: |
| Objective To investigate the expression of DRAM in breast cancer cell line MCF-7 in radiation-induced autophagy. Methods GFP-LC3 transfection method was used to observe autophagy bubble. Real time-PCR was used to detect DRAM and MAPLC3 from transcriptional and translational level, respectively. The silencing vector from gene engineering was introduced by calcium phosphate transfection. Results Compared with the control group, GFP-LC3 increased significantly after 8 Gy irradiation by immunofluorescent assay, and the level of MAP-LC3 expression was higher than control group after 8 Gy irradiation by Western blot(F=5.38,8.72,10.63,15.23,20.78 and 55.23,P<0.05). DRAM protein expression increased significantly at 2 h in the 8 Gy time-dose study, up to maximum at the 32 h(F=116.34,P<0.05). In DRAM model, the expression of LC3 and DRAM decreased significantly(F=20.36 and 40.35,P<0.05)and DRAM expression increased 8 Gy post-irradiation, but still lower than that in 8 Gy irradiation wild-type group. The LC3 expression also decreasaed 8 Gy post-irradiation(F=50.34,P<0.05). Conclusions DRAM plays an important role in irradiation-induced autophagy in breast cancer cell. DRAM might participate in the process and serve as a theoretical target for clinical treatment of breast cancer. |
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