| XU Jia-ying,JI Jun-min,JIAO Yang,WU Li,FAN Sai-jun.Effect of sanguinarine on the growth and radiosensitivity of human ovarian cancer cells[J].Chinese Journal of Radiological Medicine and Protection,2012,32(1):6-10,14 |
| Effect of sanguinarine on the growth and radiosensitivity of human ovarian cancer cells |
| Received:April 11, 2011 |
| DOI：10.3760/cma.j.issn.0254-5098.2012.01.002 |
| KeyWords:Sanguinarine Ovarian cancer Cell cycle Apoptosis Radiosensitivity |
| FundProject:教育部"长江学者和创新团队发展计划资助"项目(IRT0849);江苏省普通高校研究生科研创新计划(CX10B-0452);苏州大学优秀博士学位论文选题立项(233209749) |
| Author Name | Affiliation | E-mail | | XU Jia-ying | School of Radiation Medicine and Public Health, Soochow University, Suzhou 215123, China | | | JI Jun-min | 常州市疾病预防控制中心 | | | JIAO Yang | School of Radiation Medicine and Public Health, Soochow University, Suzhou 215123, China | | | WU Li | School of Radiation Medicine and Public Health, Soochow University, Suzhou 215123, China | | | FAN Sai-jun | School of Radiation Medicine and Public Health, Soochow University, Suzhou 215123, China | sjfan@suda.edu.cn |
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| Abstract:: |
| Objective To study the effect of sanguinarine on the growth and radiosensitivity of ovarian cancer SK-OV-3 cells. Methods Cell growth was determined by MTT and clonogenic assay. Cell cycle analysis was performed by flow cytometry assay. The cell apoptosis was analyzed by Annexin V/PI assay. Results Sanguinarine inhibited SK-OV-3 cell growth in a dose-and time-dependent fashion and its IC50 values were 3.02 and 1.11 μmol/L at 24 and 48 h, respectively. Sanguinarine also significantly triggered a sub-G1 peak, an indicator of apoptosis, and caused a G0/G1 arrest. Furthermore, the cell apoptosis induced by X-irradiation was significantly increased at 6 Gy when the cells were pre-treated with sanguinarine, in which the early apoptotic population increased from 10.28% to 43.28% (t=19.41, P<0.01) and the late apoptotic population increased from 20.26% to 30.80% (t=8.78, P<0.01). The multi-target click model was used to fit survival curves and the SER of sanguinarine treatment approached to 1.625 at the dose of D0. Conclusions Sanguinarine could inhibit SK-OV-3 cell growth by inducing apoptosis and cell cycle arrest and enhance cell radiosensitivity at low doses. |
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