| REN Rui-ping,HE Ming-yuan,DONG Chen,XIE Yue-xia,SHAO Chun-lin.Dose-response of micronuclei frequency in lymphoblast cells irradiated with different LET rays and combination of α-particles and γ-rays[J].Chinese Journal of Radiological Medicine and Protection,2012,32(1):1-5 |
| Dose-response of micronuclei frequency in lymphoblast cells irradiated with different LET rays and combination of α-particles and γ-rays |
| Received:August 27, 2011 |
| DOI：10.3760/cma.j.issn.0254-5098.2012.01.001 |
| KeyWords:α-particle γ-ray Mixed irradiation Micronucleus Dose-response |
| FundProject:国家自然科学基金(31070758;11179002) |
| Author Name | Affiliation | E-mail | | REN Rui-ping | Institute of Radiation Medicine, Fudan University, Shanghai 200032, China | | | HE Ming-yuan | Institute of Radiation Medicine, Fudan University, Shanghai 200032, China | | | DONG Chen | Institute of Radiation Medicine, Fudan University, Shanghai 200032, China | | | XIE Yue-xia | Institute of Radiation Medicine, Fudan University, Shanghai 200032, China | | | SHAO Chun-lin | Institute of Radiation Medicine, Fudan University, Shanghai 200032, China | clshao@shmu.edu.cn |
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| Abstract:: |
| Objective To investigate the dose-response of micronuclei (MN) frequency in the lymphocytes irradiated with or without combination of α-particles and γ-rays. Methods Human lymphoblast cells HMy2.CIR were irradiated with 0-1 Gy of α-particles, 0-5 Gy of γ-rays, and 0.025-0.5 Gy of α-particles followed by different doses of γ-rays, respectively. The micronuclei(MN) in the irradiated cells were measured with the cytokinesis block technique, and the dose-responses of MN were established under different irradiation conditions. Results For γ-ray irradiation, the dose-response of MN was well-fit by the linear-quadratic model with an equation Y=c+αD+βD2.For α-particle irradiation, the MN induction increased linearly with the dose less than 0.250 Gy. But when the dose of α-particles increased continually, the dose-response curve bended and could be well fit with the BaD model Y=c+αD+σ[1-exp(-δD)]exp(-βD) where radiation-induced bystander effect (RIBE) was indicated. For the combined exposure, the dose-response of MN was similar to that of γ-irradiation when the dose of α-particles was lower than 0.1 Gy, but it was similar to that of α-irradiation when the dose of α-particles was higher. When the dose of α-particles was 0.2 and 0.5 Gy, MN induced by the mixed radiation were significantly higher than the sum of corresponding irradiation alone (t=5.22-11.86, P<0.05). Conclusions The radiation damage of α-particles differs from that of γ-rays, where RIBE may be involved. The combination irradiation of α-particles and γ-rays has a synergistic effect on radiation damage of lymphoblast cells. |
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