| LI Jie-qing,SONG Xian-rang,LIU Wei,YU Jin-ming,HOU Dian-jun,MA Ya,FENG Li,QIAO Jian-wei.Study on radiosensitivity by targeting HIF-1α in human lung cancer and growth of the transplanted tumors[J].Chinese Journal of Radiological Medicine and Protection,2011,31(6):640-643 |
| Study on radiosensitivity by targeting HIF-1α in human lung cancer and growth of the transplanted tumors |
| Received:January 24, 2011 |
| DOI：10.3760/cma.j.issn.0254-5098.2011.06.005 |
| KeyWords:Hypoxia HIF-1α Lung cancer Radiosensitivity |
| FundProject:山东省自然科学基金(ZR2010CM045);山东省医药卫生科技发展计划(2009HZ078) |
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| Abstract:: |
| Objective To observe the radiosensitivity by targeting HIF-1α in human lung cancer and the effects on tumor growth in nude mice. Methods Radiosensitivity of A549 and A549/HIF-1α(-) cells were tested by clonogenic forming assay. A549/HIF-1α(-) cells and A549 cells were injected into the male BALB/C nude mice. Tumor growth was observed. The expression of HIF-1α and microvessel density were detected by immunohistochemistry method. Results SERs of HIF-1α gene silencing were 1.03 in normoxia and 1.65 in hypoxia. The sizes of tumor xenografts derived from A549/HIF-1α(-) cells were significantly reduced compared to those of the xenografts derived from A549 cells. HIF-1α protein staining result showed a dramatic decrease in tumors from A549/HIF-1α(-) mice. The microvessel densities (MVD) were 19.83 ± 4.09 in A549 group and 11.61±3.04 in A549/HIF-1α (-)group(F=15.57,P<0.05). Conclusions Hypoxia-induced radio-resistance in lung cancer A549 cells could be reversed by silencing the HIF-1α. It also retards the growth of tumor xenografts, decreases HIF-1α expression and reduces the vascularity. |
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