| HAN Guang,ZHANG Hui,XIE Cong-hua,et al.Expression of GATA-3 in mice with radiation-induced lung fibrosis[J].Chinese Journal of Radiological Medicine and Protection,2011,31(6):631-635 |
| Expression of GATA-3 in mice with radiation-induced lung fibrosis |
| Received:March 26, 2011 |
| DOI：10.3760/cma.j.issn.0254-5098.2011.06.003 |
| KeyWords:Radiation-induced lung fibrosis GATA-3 Interleukin-13 |
| FundProject:国家自然科学基金(30770653) |
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| Abstract:: |
| Objective To observe the expression of GATA-3 and IL-13 in mice with radiation-induced lung fibrosis, and to study the function of GATA-3. Methods A total of 63 C57BL/6 female mice were randomly into 2 groups, including 21 mice in control group and 42 mice in irradiated group.The thoraces of mice in irradiated group were exposed with 12 Gy of X-rays. All the mice were sacrificed at 1 h, and 1, 2, 4, 8, 16 and 24 weeks post-irradiation. The lung issues were stained by using HE and Masson methods to determine the histological changes. The expression of IL-13 in serum, and the expression of hydroxyproline and the mRNA and protein of GATA-3 in lung tissue were assayed. Results Compared with control group, there was a significant histological and pathologic change in irradiated group. The content of hydroxyproline in irradiated group was significantly higher than that in control group(Z=3.14,P<0.05).The expressions of GATA-3 and IL-13 were found in mice post-irradiation. Without causing conspicuous fibrotic pathological changes, there was a significantly elevated expression of Th2-specific transcription factor GATA-3 mRNA at 1 and 2 weeks post-irradiation (t=6.50, 6.33, P＜0.01), while the expression of IL-13 reached the maximal value in serum at 16 weeks post-irradiation(t=32.21, P＜0.01). Conclusions GATA-3 might play a role in promoting radiation-induced pulmonary fibrosis by up-regulation of expression of Th2 cytokine IL-13. |
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