| WANG Hui,FENG Jiang-bin,LU Xue,LI Yu-wen,ZHAO Hua,FAN Li,CHEN De-qing,LIU Qing-jie.Dose-effect relationship of mRNA level of human sensitive mitochondrial genes in lymphoblastoid cells induced by 60Co γ-rays[J].Chinese Journal of Radiological Medicine and Protection,2011,31(6):627-630 |
| Dose-effect relationship of mRNA level of human sensitive mitochondrial genes in lymphoblastoid cells induced by 60Co γ-rays |
| Received:April 14, 2011 |
| DOI：10.3760/cma.j.issn.0254-5098.2011.06.002 |
| KeyWords:60Co γ-rays Human lymphoblastoid cells Sensitive mitochondrial genes Gene expression Dose-effect relationship |
| FundProject:国家自然科学基金(30870749;30570551) |
| Author Name | Affiliation | | WANG Hui | National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | FENG Jiang-bin | National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | LU Xue | National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | LI Yu-wen | National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | ZHAO Hua | National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | FAN Li | National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | CHEN De-qing | National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | LIU Qing-jie | National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China |
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| Abstract:: |
| Objective To investigate the dose-effect relationship of mRNA level of sensitive mitochondrial genes in human lymphoblastoid cells induced by ionizing radiation. Methods Seven human sensitive genes, including ND3, Cyt b, COXⅠ, COXⅡ, COXⅢ, ATPase6 and ATPase8 were chosen. Changes of mRNA level of these genes were detected by RT-PCR and Real-Time PCR at 24 h after irradiation in human lymphoblastoid cells, which were exposed to 0-15 Gy of 60Co γ-rays. Results The expression of these 7 genes at mRNA level was up-regulated 24 h after irradiation in human lymphoblastoid cells. The level of gene expression of COXⅠ, which belongs to complex Ⅳ of mitochondrial respiratory chain, was most obvious, and the peak occurred after irradiation of 8 Gy, which was 13 times of the control group. A good dose-effect relationship was showed for COXⅢ gene expression at dose range of 3-10 Gy as well as 3-15 Gy for other 3 genes including ND3, ATPase6 and ATPase8. Conclusions Gene expression levels of COXⅢ, ND3, ATPase6 and ATPase8 24h post-irradiation at certain irradiation dose range could be used for radiation damage biomarkers. |
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