| LIU Xiao-dan,ZHANG Shi-meng,LI Bing,SHANG Zen-pu,XU Qin-zhi,ZHOU Ping-kun.Dose-dependent pattern of inducible mRNA expression of PIG3 gene in normal human lymphoblastoid cells by 60Co γ-rays[J].Chinese Journal of Radiological Medicine and Protection,2011,31(6):623-626 |
| Dose-dependent pattern of inducible mRNA expression of PIG3 gene in normal human lymphoblastoid cells by 60Co γ-rays |
| Received:March 09, 2011 |
| DOI：10.3760/cma.j.issn.0254-5098.2011.06.001 |
| KeyWords:Radiation injury Biodosimeter PIG3 DNA damage Gene expression Real-Time PCR |
| FundProject:国家自然科学基金杰出青年基金(30825011) |
| Author Name | Affiliation | | LIU Xiao-dan | Beijing Institute of Radiation Medicine, Beijing 100850, China | | ZHANG Shi-meng | Beijing Institute of Radiation Medicine, Beijing 100850, China | | LI Bing | Beijing Institute of Radiation Medicine, Beijing 100850, China | | SHANG Zen-pu | Beijing Institute of Radiation Medicine, Beijing 100850, China | | XU Qin-zhi | Beijing Institute of Radiation Medicine, Beijing 100850, China | | ZHOU Ping-kun | Beijing Institute of Radiation Medicine, Beijing 100850, China |
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| Abstract:: |
| Objective To investigate the dose-response pattern on the inducible expression of PIG3 mRNA in normal human lymphoblastoid AHH1 cells by 60Co γ-rays, and its possibility for developing novel radiation biodosimeter. Methods Laser confocal fluorescent microscopy was used to detect the γ-H2AX foci, a biomarker of DNA double-strand break. After irradiation with 0,1,2,4,6,8 and 10 Gy of 60Co γ-rays, AHH-1 cells were harvested at 4, 10 and 24 h post-irradiation, and subjected to total RNA extraction and detection of PIG3 mRNA expression by quantitative real-time PCR. Results PIG3 protein foci were formed in the nuclei at 30 min after irradiation, and a part of these PIG3 foci were colocalized with γH2AX foci. After irradiation, PIG3 mRNA level was enhanced with the increased time of post-irradiation, and remained an increased level at least till 24 h. The radiation-inducible expression of PIG3 mRNA was demonstrated in a dose-dependent manner. The dose-dependent range at 4 h post-irradiation was 0-6 Gy, and at 10 h and 24 h was 0-10 Gy. Conclusions PIG3 involves in the cellular response to DNA double-strand break. The dose-dependent inducible response of PIG3 mRNA expression might provide a valuable candidate for developing a novel radiation biodosimeter. |
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