| SU Ying,HE Huo-cong,WU Jun-xin,ZOU Chang-yan,LIN Ke-yu,CHEN Chao.Comparative proteomic study of nasopharyngeal carcinoma cell lines with different radiosensitivity[J].Chinese Journal of Radiological Medicine and Protection,2011,31(5):536-541 |
| Comparative proteomic study of nasopharyngeal carcinoma cell lines with different radiosensitivity |
| Received:October 21, 2010 |
| DOI：10.3760/cma.j.issn.0254-5098.2011.05.007 |
| KeyWords:Nasopharyngeal carcinoma Radiosensitivity Proteomics Mass spectrometry |
| FundProject:福建省自然科学基金(C0710004) |
| Author Name | Affiliation | | SU Ying | Laboratory of Radiobiology, Fujian Provincial Tumor Hospital, Teaching Hospital of Fujian Medical University, Fuzhou 350014, China
| | HE Huo-cong | Laboratory of Radiobiology, Fujian Provincial Tumor Hospital, Teaching Hospital of Fujian Medical University, Fuzhou 350014, China
| | WU Jun-xin | Laboratory of Radiobiology, Fujian Provincial Tumor Hospital, Teaching Hospital of Fujian Medical University, Fuzhou 350014, China
| | ZOU Chang-yan | Laboratory of Radiobiology, Fujian Provincial Tumor Hospital, Teaching Hospital of Fujian Medical University, Fuzhou 350014, China
| | LIN Ke-yu | Laboratory of Radiobiology, Fujian Provincial Tumor Hospital, Teaching Hospital of Fujian Medical University, Fuzhou 350014, China
| | CHEN Chao | Laboratory of Radiobiology, Fujian Provincial Tumor Hospital, Teaching Hospital of Fujian Medical University, Fuzhou 350014, China
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| Abstract:: |
| Objective To investigate the proteins which were associated with radiosensitivity of nasopharyngeal carcinoma(NPC) cells and could be used to predict the radiosensitivity. Methods A radioresistant subclone cell line CNE-2(R743) derived from NPC cell line CNE-2 was established. Radiosensitivity and cell cycle characteristics of CNE-2 and CNE-2(R743) were examined and compared by clonogenic survival assay and flow cytometry. The total proteins from the two cell lines were extracted and separated by two-dimensional gel electrophoresis, and the images were analyzed by Image Master 7.0 analysis software. Differentially expressed proteins in the two cell lines were identified through MALDI-TOF/TOF peptide mass fingerprint and searched in the protein sequence database. The protein expressions were confirmed by RT-PCR and Western blot. Results Totally seven differentially expressed proteins were identified, six of which were upregulated and one downregulated in the radioresistant CNE-2(R743), compared with those of CNE-2.Three out of the seven, Annexin A2, Tropomyosin 4 and GRP78 were upregulated in the CNE-2(R743), which were confirmed by Western blot and RT-PCR (t=24.22, 24.20, 29.19, P<0.05). Conclusions Differentially expressed proteins might be involved in different radiosensitivities of nasopharyngeal carcinoma cell lines, among which Annexin A2, Tropomyosin 4 and GRP78 could be the candidate biomarkers for predicting radiosensitivity of nasopharyngeal carcinoma cells. |
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