| WANG Li-zhen,YANG Min,XU Yu-ping,et al.Experimental study of 32P-CP-PLLA microparticle on human pancreatic carcinoma in nude mice[J].Chinese Journal of Radiological Medicine and Protection,2011,31(5):527-530 |
| Experimental study of 32P-CP-PLLA microparticle on human pancreatic carcinoma in nude mice |
| Received:August 27, 2010 |
| DOI：10.3760/cma.j.issn.0254-5098.2011.05.005 |
| KeyWords:Pancreatic carcinoma Nude mice 32P-CP-PLLA microparticle Therapeutic effect Toxic side-effect |
| FundProject:"863" 国家高技术研究发展计划(2007AA02Z471) |
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| Abstract:: |
| Objective To study the therapeutic and toxic effects of32P-chromic phosphate-poly (L-lactic) acid (32P-CP-PLLA) microparticle intratumoral administration into BALB/c nude mice bearing BxPc-3 human pancreatic carcinoma. Methods Twenty four nude mice bearing tumors were injected with 0, 9.3, 18.5 and 37.0 MBq 32P-CP-PLLA microparticle,respectively. The relative tumor growth rates were observed every day, and white blood cells, platelets and body weight were measured. At 14 d after administration, the tumors were removed, histological examination and immunohistochemical analysis were performed. Results The relative tumor growth rates of each treatment group was lower than 40%. Histological examination showed the degenerative necrosis at the site nearby the mircoparticle. Immunohistochemical analysis showed that the Microvessel density (MVD) and the expression of Bcl-2 in treated group were lower than those in control group. In contrast, the expression of bax in treated group were higher than those in control group. The ratio of Bcl-2/Bax protein significantly decreased in the treatment group, which were 3.83±0.43, 0.47±0.13, 1.10±0.32, 2.19±0.57 for 0, 9.3, 18.5 and 37.0 MBq 32P-CP-PLLA microparticle, respectively (t=2.36-2.77, P<0.05). MVD were 31.2±2.3, 23.8±1.5, 14.8±0.8, 11.0±1.2, respectively. Dose dependence was observed in both HE and IHC staining after 14 d treatment (t=2.30-2.57,P<0.05). Conclusions Intratumoral injection of 32P-CP-PLLA microparticle might be a safe, easy and effective radionuclide interventional therapy for pancreatic carcinoma. |
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