| ZHANG Zhuo,ZOU Li-juan,TIAN Ying-ying.Effect of elemene on radiosensitivity of A549 cells and its possible molecular mechanism[J].Chinese Journal of Radiological Medicine and Protection,2011,31(5):519-522 |
| Effect of elemene on radiosensitivity of A549 cells and its possible molecular mechanism |
| Received:August 20, 2010 |
| DOI：10.3760/cma.j.issn.0254-5098.2011.05.003 |
| KeyWords:Elemene Radiosensitivity A549 cells DNA-PKcs Bcl-2 P53 protein |
| FundProject:国家自然科学基金(30770646);辽宁省教委基金(2008T031) |
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| Abstract:: |
| Objective To investigate the effect of elemene on the radiosensitivity of A549 cells and its possible molecular mechanism. Methods The effect of radiosensitivity was detected by colony forming assay. The protein expressions of DNA-PKcs, Bcl-2 and P53 were detected with Western blot. The correlation between the protein expression of DNA-PKcs and Bcl-2,DNA-PKcs and P53 was analyzed. Results Elemene had radiosensitizing effect on A549 cells, with the SER D0 and SERDq 1.54±0.20 and 1.43±0.15, respectively for 10 μg/ml elemene, and 1.63±0.32 and 1.75±0.19, respectively for 20 μg/ml elemene. Compared with irradiation group,the expression of DNA-PKcs was reduced significantly in 10, 20 μg/ml elemene combined with radiation group (t=7.52, 8.33, P<0.05),so was for Bcl-2 (t=10.74, 11.33, P<0.05). The expression of P53 protein increased significantly (t=-9.25, 7.66,P<0.05). There was a remarkable negative correlation between the expression of DNA-PKcs and P53 (r=-0.569,P<0.05), and a remarkable positive correlation between DNA-PKcs and Bcl-2 (r=0.755, P<0.05). Conclusions Elemene has radiosensitizing effect on A549 cells, which might be related to down-regulation of DNA-PKcs gene expression, up-regulation of P53 and down-regulation of Bcl-2. |
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