| ZHENG Miao,ZHENG Kai,SUN Han-ying,SU Wen-xia,LIU Wen-li.Effects of osteoblasts on recovery of hematopoiesis and angiogenesis in acute irradiation injured mice[J].Chinese Journal of Radiological Medicine and Protection,2011,31(4):441-444 |
| Effects of osteoblasts on recovery of hematopoiesis and angiogenesis in acute irradiation injured mice |
| Received:July 28, 2010 |
| DOI：10.3760/cma.j.issn.0254-5098.2011.04.016 |
| KeyWords:Osteoblasts Irradiation injury Hematopoiesis Angiogenesis |
| FundProject:高等学校博士学科点专项科研基金(200804871044) |
| Author Name | Affiliation | E-mail | | ZHENG Miao | Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China | | | ZHENG Kai | 430030 武汉, 华中科技大学同济医学院附属同济医院综合科 | zmzk@sina.com | | SUN Han-ying | Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China | | | SU Wen-xia | Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China | | | LIU Wen-li | Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China | |
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| Abstract:: |
| Objective To explore the effects of osteoblasts on the recovery of hematopoiesis and angiogenesis in acute irradiation injury mice. Methods The femurs of 18 male BALB/c mice were used to prepare the bone marrow osteoblasts, and the rest mice were divided into 3 groups as normal group, saline group and osteoblast group. The mice in normal group received no treatment, and the other two groups were received 6.0 Gy 60Co γ-ray irradiation. After irradiation each mouse of osteoblast group was administered with 2×106 osteoblasts through tail vein injection, and equal volume saline was given to each mouse of saline group by the same way. The following factors were measured at 7, 14, 21 d after irradiation, they were the counts of peripheral blood cells and bone marrow mononuclear cells (BMMNC), the percentage of CD34+ cells in BMMNC, the histology changes and micro vascular density (MVD) of bone marrow tissue. Results The counts of peripheral blood cells, BMMNC and hematopoietic tissue area in osteoblast group were higher than those in saline group. The percentage of CD34+ cells in BMMNC and the MVD of bone marrow in osteoblast group were also higher than those in saline group at 7, 14, 21 d after irradiation(t=2.46-64.51,P<0.05). Conclusions Osteoblasts could significantly promote the recovery of hematopoiesis and angiogenesis in mice after acute irradiation injury. |
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