| YANG Bing,ZHOU Hui,ZHANG Xiao-dong,et al.Effect of irradiation on RANKL/OPG mRNA levels in mouse osteoblast in vitro[J].Chinese Journal of Radiological Medicine and Protection,2011,31(4):437-440 |
| Effect of irradiation on RANKL/OPG mRNA levels in mouse osteoblast in vitro |
| Received:August 24, 2010 |
| DOI：10.3760/cma.j.issn.0254-5098.2011.04.015 |
| KeyWords:Receptor activator of nuclear factor κB ligand Osteopregerin Radiation damage Osteoblast |
| FundProject:国家自然科学基金(30970867);天津市自然科学基金(08JCYBJC08500) |
| Author Name | Affiliation | E-mail | | YANG Bing | Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Medical College, Tianjin Key Laboratory of Nuclear Medicine, 300192 Tianjin, China | | | ZHOU Hui | 天津医科大学药学院 | | | ZHANG Xiao-dong | Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Medical College, Tianjin Key Laboratory of Nuclear Medicine, 300192 Tianjin, China | | | LIU Zheng | Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Medical College, Tianjin Key Laboratory of Nuclear Medicine, 300192 Tianjin, China | | | ZHONG Lei-lei | Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Medical College, Tianjin Key Laboratory of Nuclear Medicine, 300192 Tianjin, China | | | ZHAO Ji | Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Medical College, Tianjin Key Laboratory of Nuclear Medicine, 300192 Tianjin, China | | | FAN Fei-yue | Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Medical College, Tianjin Key Laboratory of Nuclear Medicine, 300192 Tianjin, China | | | HAN Ying | Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Medical College, Tianjin Key Laboratory of Nuclear Medicine, 300192 Tianjin, China | | | YANG Fu-jun | Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Medical College, Tianjin Key Laboratory of Nuclear Medicine, 300192 Tianjin, China | | | SUN Yuan-ming | Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Medical College, Tianjin Key Laboratory of Nuclear Medicine, 300192 Tianjin, China | yuanmings1962@163.com |
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| Abstract:: |
| Objective To study the influence of irradiation on the osteoblast function by the gene expression changes of RANKL and OPG. Methods Bone marrow stromal cells were induced to develop into early and mature osteoblasts in vitro . The characterization of osteoblasts was indentified by ALP staining. The RANKL and OPG mRNA levels in early and mature osteoblasts, which exposed to 0-4 Gy radiation were determined by RT-PCR. Results Bone marrow stromal cells had been induced to early and mature osteoblasts by osteoblast differentiation medium in vitro . In early stage of osteoblast, RANKL mRNA expression levels treated with 1 Gy irradiation was 2.83-fold higher than those other irradiation dosage groups. The RANKL mRNA expression levels of each group in early stage of osteoblasts were significantly higher than those in the mature counterpart (t=8.34-103.57,P<0.05). The ratio of RANKL/OPG mRNA was obviously greater in early osteoblast compared with the mature cells(t=2.84-20.99,P<0.05), and it was the highest in 1 Gy irradiation treated early osteoblast.Conclusions Radiation exposure of the early osteoblasts promotes osteoclasts function and results in the bone loss. |
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