| ZONG Zhao-wen,REN Yong-chuan,SHEN Yue,et al.Enhancement of distribution of dermal multipotent stem cells to bone marrow in rats of total body irradiation by platelet-derived growth factor-AA treatment[J].Chinese Journal of Radiological Medicine and Protection,2011,31(4):433-436 |
| Enhancement of distribution of dermal multipotent stem cells to bone marrow in rats of total body irradiation by platelet-derived growth factor-AA treatment |
| Received:June 07, 2010 |
| DOI：10.3760/cma.j.issn.0254-5098.2011.04.014 |
| KeyWords:Radiation injury Dermal multipotent stem cell Platelet-derived growth factor-AA |
| FundProject:创伤烧伤与复合伤国家重点实验室自主课题 (SKLZZ200817);重庆市自然科学基金重点项目(CSTC,2009BA5017) |
| Author Name | Affiliation | | ZONG Zhao-wen | Department of Trauma Surgery, Daping Hospital, Third Military Medical University, Chongqing 400042, China | | REN Yong-chuan | Department of Trauma Surgery, Daping Hospital, Third Military Medical University, Chongqing 400042, China | | SHEN Yue | Department of Trauma Surgery, Daping Hospital, Third Military Medical University, Chongqing 400042, China | | CHEN Yong-hua | Department of Trauma Surgery, Daping Hospital, Third Military Medical University, Chongqing 400042, China | | RAN Xin-ze | 第三军医大学全军复合伤研究所 国家创伤、烧伤与复合伤重点实验室 | | SHI Chun-meng | 第三军医大学全军复合伤研究所 国家创伤、烧伤与复合伤重点实验室 | | CHENG Tian-min | 第三军医大学全军复合伤研究所 国家创伤、烧伤与复合伤重点实验室 |
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| Abstract:: |
| Objective To observe whether dermal multipotent stem cells (dMSCs) treated with platelet-derived growth factor-AA(PDGF-AA)could distribute more frequently to the bone marrow in rats of total body irradiation (TBI).Methods Male dMSCs were isolated and 10 μg/L PDGF-AA was added to the culture medium and further cultured for 2 h. Then the expression of tenascin-C were examined by Western blot, and the migration ability of dMSCs was assessed in transwell chamber. The pre-treated dMSCs were transplanted by tail vein injection into female rats administered with total body irradiation, and 2 weeks after transplantation, real-time PCR was employed to measure the amount of dMSCs in bone marrow. Non-treated dMSCs served as control. Results PDGF-AA treatment increased the expression of tenascin-C in dMSCs, made (1.79±0.13)×105 cells migrate to the lower chamber under the effect of bone marrow extract, and distributed to bone marrow in TBI rats, significantly more than (1.24±0.09)×105 in non-treated dMSCs (t=8.833, P<0.01). Conclusions PDGF-AA treatment could enhance the migration ability of dMSCs and increase the amount of dMSCs in bone marrow of TBI rats after transplantation. |
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