| XU Hui-hui,WANG Li,SUI Li,GUAN Hua,WANG Yu,LIU Xiao-dan,ZHANG Shi-meng,XU Qin-zhi,WANG Xiao,ZHOU Ping-kun.Protection of vanillin derivative VND3207 on plasmid DNA damage induced by different LET ionizing radiation[J].Chinese Journal of Radiological Medicine and Protection,2011,31(4):416-419 |
| Protection of vanillin derivative VND3207 on plasmid DNA damage induced by different LET ionizing radiation |
| Received:July 20, 2010 |
| DOI：10.3760/cma.j.issn.0254-5098.2011.04.010 |
| KeyWords:Vanillin derivative DNA damage Radiation protection γ-rays Heavy ion Proton |
| FundProject:国家重点基础研究发展计划973项目(2007CB914603);国家自然科学基金杰出青年基金(30825011);国家自然科学基金青年基金(10805078) |
| Author Name | Affiliation | E-mail | | XU Hui-hui | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | WANG Li | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | SUI Li | 中国原子能科学研究院 | | | GUAN Hua | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | WANG Yu | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | LIU Xiao-dan | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | ZHANG Shi-meng | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | XU Qin-zhi | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | WANG Xiao | 中国原子能科学研究院 | | | ZHOU Ping-kun | Beijing Institute of Radiation Medicine, Beijing 100850, China | zhoupk@nic.bmi.ac.cn |
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| Abstract:: |
| Objective To evaluate the radioprotective effect of vanillin derivative VND3207 on DNA damage induced by different LET ionizing radiation. Methods The plasmid DNA in liquid was irradiated by 60Co γ-rays, proton or 7Li heavy ion with or without VND3207. The conformation changes of plasmid DNA were assessed by agarose gel electrophoresis and the quantification was done using gel imaging system. Results The DNA damage induced by proton and 7Li heavy ion was much more serious as compared with that by 60Co γ-rays, and the vanillin derivative VND3207 could efficiently decrease the DNA damage induced by all three types of irradiation sources, which was expressed as a significantly reduced ratio of open circular form (OC) of plasmid DNA. The radioprotective effect of VND3207 increased with the increasing of drug concentration. The protective efficiencies of 200 μmol/L VND3207 were 85.3%(t=3.70,P=0.033), 73.3%(t=10.58, P=0.017)and 80.4%(t=8.57,P=0.008)on DNA damage induction by 50 Gy of γ-rays, proton and 7Li heavy ion, respectively. It seemed that the radioprotection of VND3207 was more effective on DNA damage induced by high LET heavy ion than that by proton. Conclusions VND3207 has a protective effect against the genotoxicity of different LET ionizing radiation, especially for γ-rays and 7 Li heavy ion. |
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