| LI Feng-sheng,GAO Ling,LIU Li-hui,et al.Effect of ionizing radiation on invasiveness of pulmonary adenocarcinoma cells A549 and its mechanism[J].Chinese Journal of Radiological Medicine and Protection,2011,31(4):412-415 |
| Effect of ionizing radiation on invasiveness of pulmonary adenocarcinoma cells A549 and its mechanism |
| Received:April 10, 2011 |
| DOI：10.3760/cma.j.issn.0254-5098.2011.04.009 |
| KeyWords:Radiation Pulmonary adenocarcinoma Invasiveness MMP-2 STAT3 |
| FundProject:国家自然科学基金(81001216;30770640) |
| Author Name | Affiliation | E-mail | | LI Feng-sheng | Institution of Radiation Medicine,Academy of Military Medical Sciences, 100850 Beijing, China | | | GAO Ling | 中国疾病预防控制中心辐射防护与核安全医学所 | | | LIU Li-hui | Institution of Radiation Medicine,Academy of Military Medical Sciences, 100850 Beijing, China | | | DONG Bo | Institution of Radiation Medicine,Academy of Military Medical Sciences, 100850 Beijing, China | | | LUO Qing-liang | Institution of Radiation Medicine,Academy of Military Medical Sciences, 100850 Beijing, China | | | CHEN Xiao-hua | Institution of Radiation Medicine,Academy of Military Medical Sciences, 100850 Beijing, China | chenxiaohual@yahoo.com.cn |
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| Abstract:: |
| Objective To investigate the effect of ionizing radiation on the invasion of the pulmonary adenocarcinoma cell line A549. Methods The invasiveness of A549 cells irradiated with 2 and 4 Gy doses of γ-rays was detected by using transwell invasion assay. The expression levels of matrix metalloproteinase (MMP)-2 mRNA and protein and phosphorylated signal transducers and activators of transcription 3 (STAT3) protein were detected by reverse transcription PCR and Western blot. Results After irradiation with 2 or 4 Gy, the invasiveness of A549 cells increased by 200.0% (F=111.7, P<0.01) and 390.9% (F=593.7, P<0.01), respectively, compared with that in untreated A549 cells. Furthermore, the transcription and protein expression of MMP-2 24 h after irradiation and the phosphorylation of STAT3 12 h after irradiation were promoted. The irradiation-induced elevation of MMP-2 protein expression was suppressed using STAT3 phosphorylation specific inhibitor (AG490). Moreover, compared with 4 Gy of irradiation alone, treatment with 4 Gy of irradiation plus AG490 decreased the number of invasive cells by 76.1% (F=555.9, P<0.01), and the number of invasive cells in 4 Gy of irradiation plus AG490 group made up only 117.8% of that in untreated group (F=3.6, P>0.05). Conclusions Ionizing radiation could activate STAT3, which triggers the transcription of MMP-2, and then promote the invasiveness of A549 cells. |
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