| WANG Cui-lan,JIANG Qi-sheng,ZOU Yue,et al.Effects of X-rays on CC-chemokine receptor 7 expression in human lung cancer A549 cells[J].Chinese Journal of Radiological Medicine and Protection,2011,31(3):299-302 |
| Effects of X-rays on CC-chemokine receptor 7 expression in human lung cancer A549 cells |
| Received:December 02, 2010 |
| DOI：10.3760/cma.j.issn.0254-5098.2011.03.013 |
| KeyWords:Lung cancer CC-chemokine receptor 7 (CCR7) X-rays |
| FundProject: |
|
| Hits: 3528 |
| Download times: 2475 |
| Abstract:: |
| Objective To study the effects of X-ray radiation on CC-chemokine receptor 7 (CCR7) expression in human non-small cell lung cancer (NSCLC) cells. Methods Human adenocarcinoma cells of the line A549 were cultured and irradiated by X-ray at the absorbed doses of 2, 4, 6, and 8 Gy respectively by linear accelerator (with the source skin distance of 100 cm and dose rate of 442.89 cGy/min). The relative levels of CCR7 mRNA and protein expression in the A549 cells were respectively detected by real time-PCR and Western blotting 4, 12, 24, 48, and 72 h after radiation. Untreated A549 cells were used as control group. Results The expression levels of CCR7 mRNA and protein in the A549 cells began to increase since 4 h after radiation and then decreased gradually after they reached the peak. The CCR7 mRNA expression levels 72 h after radiation of the 6 and 8 Gy groups were still significantly higher than those of the control group (t=6.75-7.26,both P <0.01), and the CCR7 protein expression levels of the 2 and 6 Gy group were still significantly higher than those of the control group(t=11.13-14.17,both P <0.01). Then the CCR7 protein expression levels of the 4 and 8 Gy groups decreased to the control group level 48 and 72 h after radiation respectively. Conclusions The CCR7 mRNA and protein expression levels in the NSCLC cells increase after X-ray irradiation, which may be correlated with the promotion of proliferation and metastasis of NSCLC cells by X-ray irradiation at a certain dose. |
| HTML View Full Text View/Add Comment Download reader |
| Close |
|
|
|
