| QI Xue-song,LV Hui-min,WANG Chun-yan,et al.Dose-effect relationship and time-effect relationship of T cell receptor gene mutation induced by γ-rays in human lymphocytes of peripheral blood[J].Chinese Journal of Radiological Medicine and Protection,2011,31(3):286-289 |
| Dose-effect relationship and time-effect relationship of T cell receptor gene mutation induced by γ-rays in human lymphocytes of peripheral blood |
| Received:October 21, 2010 |
| DOI：10.3760/cma.j.issn.0254-5098.2011.03.010 |
| KeyWords:γ-rays T cell receptor (TCR) Dose-effect relationship Time-effect relationship Biological dosimeter |
| FundProject:卫生行业科研专项(200802018) |
| Author Name | Affiliation | | QI Xue-song | National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | LV Hui-min | National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | WANG Chun-yan | National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | ZHANG Wei | National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | HAO Shu-xia | National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | GOU Qiao | National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | TONG Peng | National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | LIU Qing-jie | National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | SU Xu | National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China |
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| Abstract:: |
| Objective To study the dose-effect relationship and time-effect relationship of T cell receptor (TCR) gene mutation induced by γ-rays in lymphocytes of human peripheral blood. Methods Samples of peripheral blood were collected from 10 healthy adults and lymphocytes were separated. Four samples from males used to fit time-effect curve were exposed to γ-rays at the doses of 0, 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5, 4.0, and 5.0 Gy, respectively, and 6 samples from 3 males and 3 females used to fit dose-effect curves were exposed to γ-rays of the dose of 2 Gy. Flow cytometry was used to detect the mutation frequency of TCR gene (TCR MF). Radiation dose-effect curves and time-effect curves were fitted and optimal mathematical models were selected respectively. Results The optimal mathematical model for radiation dose-effect was quadratic equation model: TCR MF=92.14+22.61 D 2(R2adj=0.65). The optimal mathematical model for radiation time-effect was quadratic polynomial equation model: TCR MF=3.74+743.66 T +308.64 T 2(R2adj=0.79). Conclusions TCR MF is increased as the γ-ray irradiation dose increases within the range of 0-5 Gy, and TCR MF is increased with the lapse of time within the range of 4 days after γ-ray radiation. |
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