| XU Zheng-min,YAN Jia-cheng,LI Xian-fu,TAN Bang-xian,TANG Zhong,MAO Ming,CHENG Ji-bing,WANG Han-yan,TANG Hua-ying,CHEN Jian-ye.Different roles of total flavonoids of astragalus on human normal mesenchymal stem cells and hepatoma cells in radiation protection[J].Chinese Journal of Radiological Medicine and Protection,2011,31(3):282-285 |
| Different roles of total flavonoids of astragalus on human normal mesenchymal stem cells and hepatoma cells in radiation protection |
| Received:May 21, 2010 |
| DOI：10.3760/cma.j.issn.0254-5098.2011.03.009 |
| KeyWords:Apoptosis Radiation protection Total flavonoids of Astragalus (TFA) HepG-2 Human normal mesenchymal stem cells |
| FundProject:四川省科技厅重点项目(05SG1859);四川省科技厅应用基础项目(2006J13-031) |
| Author Name | Affiliation | | XU Zheng-min | Department of Biochemistry, North Sichuan Medical College, Nanchong 637000, China | | YAN Jia-cheng | Department of Biochemistry, North Sichuan Medical College, Nanchong 637000, China | | LI Xian-fu | Department of Biochemistry, North Sichuan Medical College, Nanchong 637000, China | | TAN Bang-xian | Department of Biochemistry, North Sichuan Medical College, Nanchong 637000, China | | TANG Zhong | Department of Biochemistry, North Sichuan Medical College, Nanchong 637000, China | | MAO Ming | Department of Biochemistry, North Sichuan Medical College, Nanchong 637000, China | | CHENG Ji-bing | Department of Biochemistry, North Sichuan Medical College, Nanchong 637000, China | | WANG Han-yan | Department of Biochemistry, North Sichuan Medical College, Nanchong 637000, China | | TANG Hua-ying | Department of Biochemistry, North Sichuan Medical College, Nanchong 637000, China | | CHEN Jian-ye | Department of Biochemistry, North Sichuan Medical College, Nanchong 637000, China |
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| Abstract:: |
| Objective To investigate the different radioprotective effects of total flavonoids of Astragalus (TFA) on human normal mesenchymal stem cells(hMSCs)and hepatoma cells injured by 60Co γ-ray radiation. Methods hMSCs and HepG-2 cells were cultured and randomly divided into TFA-treated and untreated groups. The cells of different groups were irradiated with 60Co γ -rays at the dose of 6 Gy. MTT method was utilized to detect the survival rates of the hMSCs and HepG-2 cells pretreated or untreated with TFA before irradiation. Cell clone formation test was used to measure the cellular radiosensitivity. The apoptosis rates of different groups were determined by flow cytometer assay. The expression rates of the apoptosis-promoting proteins Fas and Bax and the apoptosis-inhibiting protein Bcl-2 were analyzed by Western blotting. Results MTT showed that the survival rates of hMSCs pretreated by TFA were 1.15-1.95 times higher than that of the pure irradiation group. On the contrary, the survival rates of the TFA pretreated HepG-2 cells were only 0.53-0.23 times that of the pure irradiation group. There was a good dose-effect relationship between the cell survival rate and the TFA concentration. Cell clone formation rate indicated that combined treatment of TFA and radiation inhibited the cell proliferation more effectively than single TFA or pure radiation.Flow cytometry showed that 6, 24 and, 48 h post-irradiation to 6 Gy, the apoptosis rates of the hMSCs were 23.3%, 11.2%, and 2.9%, respectively in the TFA pretreated group and were 29.3%, 24.9%, and 13.6% in the pure radiation group. However, the apoptosis rates of the HepG-2 cells at 6, 24, and 48 h post-irradiation to 6 Gy were 11.6%, 17.3%, and 20.1%, respectively in the TFA pretreated group and were 6.9%, 9.3%, and 15.8%, respectively in the direct radiation group. Western blotting showed that the expression levels of Fas and Bax proteins in the HepG-2 cells were significantly higher in the TFA pretreated group than in the pure radiation group. On the contrary, the expression level of the apoptosis inhibiting protein Bcl-2 was significantly lower in the TFA pretreated group than in the pure radiation group. Conclusions TFA has obvious effects of radiological protection on human hMSCs and has no effects of radiological protection but effects of apoptosis enhancement on hepatoma cells. The promotion of apoptosis of TFA on hepatoma cells is primarily through increasing the expression of apoptotic proteins such as Fas and Bax and reducing the expression of anti-apoptotic protein Bcl-2. |
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