| GAO Ling,LI Feng-sheng,DONG Bo,et al.Effects of signal transducer and activator of transcription 3 RNAi on content of reactive oxygen species and DNA damage in glioma cell[J].Chinese Journal of Radiological Medicine and Protection,2011,31(3):269-272 |
| Effects of signal transducer and activator of transcription 3 RNAi on content of reactive oxygen species and DNA damage in glioma cell |
| Received:October 29, 2010 |
| DOI：10.3760/cma.j.issn.0254-5098.2011.03.006 |
| KeyWords:STAT3 siRNA Glioma Reactive oxygen species DNA damage |
| FundProject:国家自然科学基金(30770640;81001216) |
| Author Name | Affiliation | | GAO Ling | Institution of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | LI Feng-sheng | Institution of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | DONG Bo | Institution of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | LIU Li-hui | Institution of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | LIU Qing-jie | 中国疾病预防控制中心辐射防护与核安全医学所 | | CHEN Xiao-hua | Institution of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | MAO Bing-zhi | Institution of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China |
|
| Hits: 4091 |
| Download times: 2953 |
| Abstract:: |
| Objective To investigate the effects of s ignal transducer and activator of transcription 3 (STAT3) RNAi on the content of reactive oxygen species (ROS) and the DNA damage in glioma cells. Methods Glioma cells of the line U251 cells were cultured and transfected with STAT3 RNAi plasmid(pSilencer2.1-STAT3, STAT3 group) and pSilencer2.1-GFP (GFP control group) respectively. Part of the U251 cells were irradiated with γ-rays of 60Co as positive control group of smear phenomenon. The levels of ROS and malondialdehyde (MDA) in the cells were detected 24, 48, and 72 h later by flow cytometry and fluorescence chamoluminescence analyzer, respectively. The DNA damage in the transfected U251 cells was examined by using single cell gel electrophoresis assay, and the cell cycle distribution was examined using FACS PI staining 12, 24, and 36 h later. Results At 24 h after the transfection, the ROS level of the siSTAT3-transfected cells was 8.91 times that of the control group (F =89.296, P <0.05), and returned to the normal level 48 h later. There were not significant differences in the MDA level of the cells 24, 48, and 72 h later between the siSTAT3 group and siGFP group. Compared with the 8 Gy irradiation positive group with obvious smear phenomenon, smear phenomenon was shown in part of the cells in the siSTAT3 group 6 h later, became less 12 h later, and disappeared completely 24 h later. Compared with the control group, lag of S stage rate was 17.22% and the lag of G2/M stage rate was 6.4% 12 h later in the siSTAT-transfected group, and the G0/G1 stage lag rate was 18.44% 24 h later, and the lag of S stage rate was 17.99% 36 h later. Conclusions Inhibition of STAT3 results in the change of oxidoreduction status in glioma cells, as well as damage and reparation of DNA. |
| HTML View Full Text View/Add Comment Download reader |
| Close |
|
|
|
