| ZHANG Zhuo,ZOU Li-juan,TIAN Ying-ying.Screening radiosensitizing-related genes mediated by elemene in lung adenocarcinoma A549 cells by using gene chip[J].Chinese Journal of Radiological Medicine and Protection,2011,31(3):264-268 |
| Screening radiosensitizing-related genes mediated by elemene in lung adenocarcinoma A549 cells by using gene chip |
| Received:September 16, 2010 |
| DOI：10.3760/cma.j.issn.0254-5098.2011.03.005 |
| KeyWords:Gene chip Elemene Radioensitizing Lung adenocarcinoma A549 cells |
| FundProject:国家自然科学基金(30770646); 辽宁省教委基金(2008T031) |
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| Abstract:: |
| Objective To screen radiosensitizing-related genes mediated by elemene in lung adenocarcinoma A549 cells by using gene chip. Methods MTT test was used to calculate the IC50 of elemene.1The effect of radiosensitivity was detected by colony forming assay. A549 cells were divided into 2 groups: radiation group and radiation+elemene group. Oligonucleotide chip was used to screen the gene expression changes of A549 cells from these 2 groups. The up-regulated gene Egr-1 and the down-regulated gene CyclinD1 were selected to undergo RT-PCR so as to confirm the reliability of the result. Results MTT test showed the elemene inhibited the proliferation of the A549 cells dose-dependently. The IC5 0 value of elemene on the A549 cells was 120 mg/L. 210 mg/L elemene had radiosensiting effect on A549 cells. The values of SERD0 and SERDq obtained from the survival curve were (1.54±0.20) and (1.43±0.15) respectively. Gene chip screened 122 differentially-expressed genes, including 89 up-regulated genes and 33 down-regulated genes. 3These altered genes could be related to cell structure, substance metabolism, cell proliferation, cell differentiation, signal transduction, material transport, DNA repair, apoptosis, immune response and so forth. The RT-PCR results of Egr-1 and Cyclin D1 were consistent with the genen chip analysis. Conclusions The mechanism of elemene enhancing the radiosensitivity of lung adenocarcinoma A549 cells is the result of participation and collaboration of multiple genes. Further study of the newly-discovered differentially-expressed gene helps find out new radiosensitizational targets of elemene. |
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