| PAN Bin,ZENG Ling-yu,CHENG Hai,et al.Effects of X-rays and γ-rays on reconstitution of hematopoiesis and immunity after allogeneic bone marrow transplantation[J].Chinese Journal of Radiological Medicine and Protection,2011,31(3):260-263 |
| Effects of X-rays and γ-rays on reconstitution of hematopoiesis and immunity after allogeneic bone marrow transplantation |
| Received:June 14, 2010 |
| DOI：10.3760/cma.j.issn.0254-5098.2011.03.004 |
| KeyWords:X-rays γ-rays Allogeneic hematopoietic stem cell transplantation Immune reconstitution |
| FundProject:国家自然科学基金(30971281) |
| Author Name | Affiliation | | PAN Bin | Department of Hematology, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, China | | ZENG Ling-yu | Department of Hematology, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, China | | CHENG Hai | Department of Hematology, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, China | | SONG Guo-liang | Department of Hematology, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, China | | JIA Lu | Department of Hematology, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, China | | YAN Zhi-ling | Department of Hematology, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, China | | CHEN Chong | Department of Hematology, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, China | | XU Kai-lin | Department of Hematology, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, China |
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| Abstract:: |
| Objective To determine the conditioning regimen suitable for mice allogeneic hematopoietic stem cell transplantation (allo-HSCT). Methods Twelve BALB/c mice were randomly divided into 2 equal groups to undergo X-ray irradiation by linear accelerator at the dose of 7.0 Gy (pure X-ray group) or 60Co source irradiation at the dose of 7.0 Gy (pure γ-ray group). Thirty mice were randomly divided into 2 equal groups to undergo X-ray irradiation and then infusion of bone marrow from donor mice via caudal vein (X-ray+transplantation group) or γ-ray and then infusion of bone marrow via caudal vein (γ-ray+transplantation group). 3, 5, 7, 10, 15, 20, and 30 d later peripheral blood samples were collected to calculate the number of white blood cells (WBCs) and detect the chimeric rates of lymphocytes by flow cytometry. 5, 10, and 20 d after irradiation 15 mice were killed with their lung, liver, small intestine, spleen, and femurs taken out to undergo pathological examination. Results The survival rates during the period 5-15 days of the γ-ray+transplantation group were all significantly higher than those of the X-ray+transplantation group. The pathological changes of organs of the X-ray+transplantation group were all more severe than those of the γ-ray+transplantation group. Since the fifth day after transplantation cells originating from the donor began to appear in the peripheral blood. The chimeric rate of the γ-ray+transplantation group 10 days after transplantation was (95.53±2.57) %. The chimeric rates 5, 10, and 20 days after transplantation of the γ-ray+transplantation group were all significantly higher than those of the X-ray+transplantation group (t=15.263,3.256, P <0.05). The WBC count of both irradiation groups decreased to the lowest level 5 d later and began to increase 10 days after transplantation and the WBC counts of the γ-ray+transplantation group 10 and 20 days after transplantation were both significantly higher than those of the X-ray+transplantation group (t=3.624, 6.695, P <0.05). The chimeric rats of the peripheral lymphocytes 10 and 20 days after transplantation of the γ-ray+transplantation group were both significantly higher than those of the X-ray+transplantation group (t=12.317, 8.295, P <0.05).The homogeneity rate of transplantation of the γ-ray+transplantation group was better than that of the X- ray+transplantation group. Conclusions As a conditioning regimen in allogeneic hematopoietic stem cell transplantation γ-ray irradiation causes milder injury and accelerated reconstitution of hematopoiesis and immunity, in comparison with X-ray irradiation. |
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