| XING Shuang,HU Zhi-qing,LI Ming,et al.Experimental therapeutic effect of combined cytokines on beagle dogs exposed to 4.5 Gy γ-rays[J].Chinese Journal of Radiological Medicine and Protection,2010,30(3):243-246 |
| Experimental therapeutic effect of combined cytokines on beagle dogs exposed to 4.5 Gy γ-rays |
| Received:December 24, 2009 |
| DOI：10.3760/cma.j.issn.0254-5098.2010.03.003 |
| KeyWords:Cytokine Acute radiation sickness Recombinant human interleukin-11 Recombinant human G-CSF Recombinant human interleukin-2 Beagles |
| FundProject:国防科工委"十一五"核科学基础研究重点项目(A3620060130) |
| Author Name | Affiliation | E-mail | | XING Shuang | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | HU Zhi-qing | 苏州大学医学 | | | LI Ming | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | HUANG Hai-xiao | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | OU Hong-ling | 第二炮兵总医院 | | | ZHAO Jian-zhi | 苏州大学附属第一医院 | | | XIONG Guo-lin | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | XIE Ling | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | ZHAO Zhen-hu | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | WANG Ning | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | WANG Jin-xiang | 苏州大学医学 | | | ZHANG Ri | 苏州大学附属第一医院 | | | MIAO Jing-cheng | 苏州大学医学 | | | ZHU Nan-kang | 苏州大学医学 | | | CONG Yu-wen | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | LUO Qing-liang | 100850 北京, 军事医学科学院放射与辐射研究所 | | | ZHANG Xue-guang | 苏州大学医学 | xgzh@ yahoo. com. cn |
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| Abstract:: |
| Objective To evaluate the effects of combined administration of recombinant human interleukin-11 (rhIL-11), recombinant human G-CSF (rhG-CSF) and recombinant human interleukin-2 (rhIL-2) on acute radiation sickness (ARS) beagles. Methods Sixteen beagle were irradiated with 4.5 Gy 60Coγ-rays to establish ARS models, and were divided into irradiation control group, supportive care group and combined cytokines treatment group. After irradiation irradiation control group was given no treatment, the dogs in supportive care group received purely symptomatic treatment, while combined cytokines treatment group received rhIL-11 50μg/(kg ?d) and rhG-CSF 10μg/(kg ?d) subcutaneously(0-14 d) and rhIL-2 1?106 U/d (29-43 d) besides symptomatic treatment. Manifestation and characteristics of ARS beagles were observed, and the survival time were recorded. At last, post-mortem examination and histological examination were performed. Results All animals underwent nausea, diarrhea and fever. After irradiation, all animals in irradiation control group died in two weeks, and the mean survival time was 12.7 d, while only one died at 33 d in supportive care group. All dogs in combined cytokine group survived at 45 day after exposure, and their haematopoiesis and gastrointestinal tract were recovered. Conclusions Combination of rhIL-11 rhG-CSF rhIL-2 treatment could be significantly effective on ARS beagles irradiated by 4.5 Gy 60Coγ-rays, which could accelerate injured haemotopoiesis and intestinal tract recovery, increase the survival rate and improve the life quality of animals. |
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