| ZHANG Hong-bo,ZHANG Hong-yan,WANG Hong-mei,LUO Wen-guang,CHEN Long-hua.Effect of N-Ras by RNA interference on radiosensitivity of hepatoma carcinoma cell MHCC97-H line[J].Chinese Journal of Radiological Medicine and Protection,2011,31(3):252-255 |
| Effect of N-Ras by RNA interference on radiosensitivity of hepatoma carcinoma cell MHCC97-H line |
| Received:October 27, 2010 |
| DOI：10.3760/cma.j.issn.0254-5098.2011.03.002 |
| KeyWords:Radiosensitivity N-Ras gene RNA interference Hepatoma carcinoma |
| FundProject:国家自然科学基金(30772530);安徽省卫生厅医学科研资助项目(09B109) |
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| Abstract:: |
| Objective To investigate the radiosensitivity of silencing N-Ras by RNA interference in hepatoma carcinoma cell MHCC-97. Methods N-Ras RNA interference(RNAi) vector was constructed by using pcDNA 6.2-GW/EmGFP-mir plamid. The RNAi effect was detected by RT-PCR,Western bolt,immunohistochemisty and MTT method.Survival curve for each cell line were obtained by measuring the clone forming abilities of irradiated cell populations. Results After silencing the N-Ras by RNAi,The expression level of N-Ras mRNA,N-Ras protein, immunohistochemisty were decreased 96.9%±0.159%(t=40.377, P <0.05),89.8%±0.012%(t=31.595, P <0.05), 90%, respectively.and The survival of hepatoma carcinoma cell MHCC-97 line were inhibited 21.9%(F =4.63, P <0.05).Which have significant difference in statistics.The SER of hepatoma carcinoma cell MHCC-97 line after interference was 1.15. Conclusions RNAi targeting silence N-Ras may increase the radiosensitivity of hepatoma carcinoma cell MHCC-97 line. |
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