| REN Jun-hui,PENG Rui-yun,ZHANG Jing,et al.Prevention effects of AduoLa Fuzhenglin on brain injury induced by microwave radiation in rats[J].Chinese Journal of Radiological Medicine and Protection,2011,31(2):190-193 |
| Prevention effects of AduoLa Fuzhenglin on brain injury induced by microwave radiation in rats |
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| DOI：10.3760/cma.j.issn.0254-5098.2011.02.018 |
| KeyWords:AduoLa Fuzhenglin Microwave Rat Brain injury Prevention |
| FundProject:国家科技重大专项(2008ZXJ09004-019) |
| Author Name | Affiliation | | REN Jun-hui | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | PENG Rui-yun | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | ZHANG Jing | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | LI Jing | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | GAO Ya-bing | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | WANG Shui-ming | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | DONG Ji | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | ZUO Hong-yan | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | SU Zhen-tao | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | ZHOU Hong-mei | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China |
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| Abstract:: |
| Objective To study the prevention effects of AduoLa Fuzhenglin (ADL) on the brain injury induced by microwave radiation in rats. Methods A total of 140 male Wistar rats were divided randomly into 5 groups, including control group, microwave exposed group, low dosage (0.75 g ·kg-1 ·d-1 ) group, middle dosage (1.5 g ·kg-1 ·d-1) group and high dosage(3 g ·kg-1 ·d-1) group. Rats in three ADL groups were lavaged with ADL per day for 2 weeks before radiation. After administration, rats were exposed to microwave at 30 mW/cm2 for 15 min. The abilities of learning and memory were detected by Morris water maze, and the contents of amino acids neurotransmitter of hippocampus were detected by HPLC, then the histology and ultrastructure of hippocampus were observed with light and electron microscope at 6 h, 7 and 14 d after exposure. Results The abilities of learning and memory were declined (F=0.000-0.043, P<0.05) from 6 h to 7 d after exposure, and the contents of four kinds of amino acid neurotransmitter in hippocampus were decreased, of which GLU, GLY and GABA were decreased significantly (F=0.000-0.007, P<0.01) at 6 h after exposure, then tissue edema, neuronal degeneration, neuron mitochondria swelling and cavitation, endocytoplasmic reticulum broaden, synaptic cleft blurred, and perivascular space widen were found in the hippocampus at 6 h and 7 d after exposure. The changes in low dosage group were similar to those of the radiation group. However, in middle and high dosage groups, the abilities of learning and memory were normal to some extent with the significant differences compared to the radiation group from 6 h to 7 d after exposure(F=0.015-0.028, P<0.05). The contents of four kinds of amino acid neurotransmitter were not decreased, especially GLU contents close to normal level. There were significant differences between middle and high dosage groups and radiation group at 6 h after exposure (F=0.000-0.042, P<0.05). Moreover, no obvious injury in the hippocampus was observed in middle and high dosage groups at 6 h and 7 d after exposure. Conclusions Exposure to 30 mW/cm2 microwave radiation could decrease the abilities of learning and memory, induce amino acid neurotransmitter turbulence, and injure the histology and ultrastructure of hippocampus. ADL at the dosages of 1.5 and 3 g ·kg-1 ·d-1 would have preventive effects on the injury induced by microwave exposure. The concentration of 1.5 g ·kg-1 ·d-1 of ADL might be the effective dosage to prevent the brain damage after microwave exposure. |
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