| WANG Lu,ZOU Yue,JIANG Qi-sheng,et al.Effects of X-ray irradiation on expression of Pokemon gene in A549 cells of human lung adenocarcinoma[J].Chinese Journal of Radiological Medicine and Protection,2011,31(2):177-179 |
| Effects of X-ray irradiation on expression of Pokemon gene in A549 cells of human lung adenocarcinoma |
| Received:October 12, 2010 |
| DOI：10.3760/cma.j.issn.0254-5098.2011.02.015 |
| KeyWords:Lung cancer Pokemon gene A549 cells X-rays Radiation |
| FundProject:全军"十一五"卫生科研规划资助项目(06MA035);国家自然科学基金(30271479) |
| Author Name | Affiliation | | WANG Lu | School of Radiation Medicine and Public Health, Medical College of Soochow University, Suzhou 215123,China | | ZOU Yue | School of Radiation Medicine and Public Health, Medical College of Soochow University, Suzhou 215123,China | | JIANG Qi-sheng | School of Radiation Medicine and Public Health, Medical College of Soochow University, Suzhou 215123,China | | LI Wei | School of Radiation Medicine and Public Health, Medical College of Soochow University, Suzhou 215123,China | | SONG Xiu-jun | School of Radiation Medicine and Public Health, Medical College of Soochow University, Suzhou 215123,China | | ZHOU Xiang-yan | School of Radiation Medicine and Public Health, Medical College of Soochow University, Suzhou 215123,China | | WANG Cui-lan | School of Radiation Medicine and Public Health, Medical College of Soochow University, Suzhou 215123,China |
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| Abstract:: |
| Objective To study the dose-and time-effects of X-ray irradiation on the expression of Pokemon gene in A549 cells of human lung adenocarcinoma. Methods A549 cells were cultured in vitro and exposed to X-rays with the doses of 2, 4, 6 and 8 Gy, respectively. Untreated A549 cells were used as control group. The relative levels of Pokemon mRNA expression in the cells were detected by using quantitative real-time PCR at 2, 4, 8, 12, 24, 48 and 72 h after irradiation. Results The Pokemon mRNA expression levels decreased in the early period after irradiation (except 2 and 4 h after irradiation in 2 Gy group) and then increased in the later stage (48 h after irradiation) with significant statistical differences at the most time points in comparison with the control group(t=3.40-154.76, P<0.05).Conclusions Higher doses of X-rays may degrade the expression of Pokemon mRNA in the human A549 cells and induce apoptosis in the early period, but also may upgrade its expression in the later period, which might be correlated with the cell cycle regulation and DNA damage repair in the A549 cells. |
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