| GUO Ya,ZHU Xiao-dong,QU Song,et al.Radioresistance-related signaling pathways in nasopharyngeal carcinoma cells[J].Chinese Journal of Radiological Medicine and Protection,2011,31(2):167-171 |
| Radioresistance-related signaling pathways in nasopharyngeal carcinoma cells |
| Received:April 23, 2010 |
| DOI：10.3760/cma.j.issn.0254-5098.2011.02.013 |
| KeyWords:Nasopharyngeal carcinoma Signaling pathway Radioresistance Gene chip |
| FundProject:国家自然科学基金(30860329);广西壮族自治区自然科学基金(桂科自0832229);广西2010年研究生教育创新计划(2010105981002M214);广西大型仪器协作共用网测试补助项目(851-2009-055) |
| Author Name | Affiliation | | GUO Ya | Cancer Hospital of Guangxi Medical University, Cancer Institute of Guangxi Zhuang Autonomous Region, Nanning 530021, China | | ZHU Xiao-dong | Cancer Hospital of Guangxi Medical University, Cancer Institute of Guangxi Zhuang Autonomous Region, Nanning 530021, China | | QU Song | Cancer Hospital of Guangxi Medical University, Cancer Institute of Guangxi Zhuang Autonomous Region, Nanning 530021, China | | SU Fang | Cancer Hospital of Guangxi Medical University, Cancer Institute of Guangxi Zhuang Autonomous Region, Nanning 530021, China | | WANG Qi | Cancer Hospital of Guangxi Medical University, Cancer Institute of Guangxi Zhuang Autonomous Region, Nanning 530021, China | | ZHANG Wei | Cancer Hospital of Guangxi Medical University, Cancer Institute of Guangxi Zhuang Autonomous Region, Nanning 530021, China |
|
| Hits: 3964 |
| Download times: 2747 |
| Abstract:: |
| Objective To study the difference of gene expression profile between the radioresistant human nasopharyngeal carcinoma cell line CNE-2R and CNE-2, and to screen the signaling pathway associated with radioresistance of nasopharyngeal carcinoma. Methods The radioresistant nasopharyngeal carcinoma cell line CNE-2R was constructed from the original cell line CNE-2. CNE-2R and CNE-2 cells were cultured and administered with 60Co γ-ray irradiation at the dose of 400 cGy for 15 times. Human-6v 3.0 whole genome expression profile was used to screen the differentially expressed genes. Bioinformatic analysis was used to identify the pathways related to radioresistance. Results The number of the differentially expressed genes that were found in these 2 experiments was 374. The Kegg pathway and Biocarta pathway analysis of the differentially expressed genes showed the biological importance of Toll-like receptor signaling pathway and IL-1R-mediated signal transduction pathway to the radioresistance of the CNE-2R cells and the significant differences of 13 genes in these 2 pathways, including JUN, MYD88, CCL5, CXCL10, STAT1, LY96, FOS, CCL3, IL-6, IL-8, IL-1α, IL-1β, and IRAK2 (t=13.47-66.57,P < 0.05). Conclusions Toll-like receptor signaling pathway and IL-1R-mediated signal transduction pathway might be related to the occurrence of radioresistance. |
| HTML View Full Text View/Add Comment Download reader |
| Close |
|
|
|
