| NI Guan-ying,WU Ning,GUO Hai-zhuo,et al.Effects of ionizing radiation on expression of P21 protein in Jurkat cell line and p21 gene in thymocytes and splenocytes of mice[J].Chinese Journal of Radiological Medicine and Protection,2011,31(2):134-137 |
| Effects of ionizing radiation on expression of P21 protein in Jurkat cell line and p21 gene in thymocytes and splenocytes of mice |
| Received:December 01, 2010 |
| DOI：10.3760/cma.j.issn.0254-5098.2011.02.005 |
| KeyWords:Ionizing radiation Jurkat cell line P21 protein p21 gene Thymocyte Splenocyte |
| FundProject:卫生行业科研专项(200802018) |
| Author Name | Affiliation | | NI Guan-ying | PET-CT Center, Jilin Tumor Hospital, Changchun 130021, China | | WU Ning | PET-CT Center, Jilin Tumor Hospital, Changchun 130021, China | | GUO Hai-zhuo | PET-CT Center, Jilin Tumor Hospital, Changchun 130021, China | | JIN Shun-zi | PET-CT Center, Jilin Tumor Hospital, Changchun 130021, China |
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| Abstract:: |
| Objective To investigate the effects of ionizing radiation on the expression of P21 protein in Jurkat cell line and p21 gene in thymocytes and splenocytes of mice. Methods Flow cytometry (FCM) was used to analyze the expression of P21 protein in Jurkat cells at 12 and 24 h after irradiation to 0, 0.5, 1.0, 2.0, 4.0, and 6.0 Gy. Real-time PCR was used to detect the expression of p21 gene in thymocytes and splenocytes of mice at 4 and 24 h after irradiation to 0, 0.5, 1.0, 2.0, 4.0, and 6.0 Gy. Multi-staining was used to analyze the micronucleus rates of Rct in bone marrow. Results The expressions of P21 protein were increased in a dose-dependent manner during 0.5-4.0 Gy( t =-24.23--3.96, P <0.05), but decreased at 6.0 Gy at 12 and 24 h post-irradiation( t =-11.19,-14.50, P <0.05). The expressions of p21 gene in both thymocytes and splenocytes of mice were increased in dose-dependent manner in the range of 0-6.0 Gy(including 6.0 Gy)( t =-29.96-8.80, P <0.05), and reached to the peak at 6.0 Gy at 4 and 24 h post-irradiation( t =-11.84--3.42, P <0.05), except thymocytes at 4 h and 1.0 Gy post-irradiation( t =-3.42, P >0.05). Conclusions The expressions of P21 protein and p21 gene could be increased by X-ray irradiation, which shows good dose-dependent manners in certain range of dose. |
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