| TIAN Mei,PAN Yan,LIU Jian-xiang,RUAN Jian-lei,SU Xu.Human lymphocyte damage and phosphorylation of H2AX and ATM induced by γ-rays[J].Chinese Journal of Radiological Medicine and Protection,2011,31(2):126-129 |
| Human lymphocyte damage and phosphorylation of H2AX and ATM induced by γ-rays |
| Received:December 07, 2010 |
| DOI：10.3760/cma.j.issn.0254-5098.2011.02.003 |
| KeyWords:60Co γ-rays Lymphocyte γ-H2AX ATM phosphorylation Micronucleus |
| FundProject:卫生行业科研专项(200802018) |
| Author Name | Affiliation | | TIAN Mei | National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | PAN Yan | National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | LIU Jian-xiang | National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | RUAN Jian-lei | National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | SU Xu | National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China |
|
| Hits: 5061 |
| Download times: 2889 |
| Abstract:: |
| Objective To investigate 60Co γ-ray induced damage in lymphocytes and the relationship between doses of 60Co γ-ray irradiation and the levels of phosphorylated H2AX and ATM. Methods Cells were irradiated with 60Co γ-rays in the range of 0-8 Gy. The levels of phosphorylated H2AX and ATM were detected by Western blot and FACScan, respectively. The micronucleus(MN) was analyzed by CB method to evaluate DNA damage. Results FACScan results showed the dose-effect relationship of γ-H2AX expression were linear-square at 0.5 h post-irradiation to different doses, and the fitting curve was shown as Y=3.96+11.29D-0.45D2. The level of phosphorylated ATM (p-ATM) was not changed significantly by using the same method. Western blot showed that p-ATM protein expression was significantly increased after irradiation compared with sham-irradiated group. The MN assay which represented DNA damage was sensitive to different doses. Conclusions γ-ray irradiation could induce the phosphorylation of H2AX and ATM, which may play an important role in indicating DNA damage. Both of H2AX and ATM have the potential as sensitive biomarker and biodosimeter for radiation damage. |
| HTML View Full Text View/Add Comment Download reader |
| Close |
|
|
|
