| LIU Li-bo,ZHANG Jian,LIU Yu-guang,et al.Correlation analysis on MN-RET from peripheral blood and MN-PCE from bone marrow in mice following exposure to irradiation[J].Chinese Journal of Radiological Medicine and Protection,2011,31(2):122-125 |
| Correlation analysis on MN-RET from peripheral blood and MN-PCE from bone marrow in mice following exposure to irradiation |
| Received:December 01, 2010 |
| DOI：10.3760/cma.j.issn.0254-5098.2011.02.002 |
| KeyWords:Radiation Mice Reticulocyte micronucleus Polychromatic erythrocyte micronucleus Flow cytometry Biodosimeter |
| FundProject:卫生行业科研专项(200802018) |
| Author Name | Affiliation | | LIU Li-bo | School of Public Health, Jilin University, Changchun 130021,China | | ZHANG Jian | School of Public Health, Jilin University, Changchun 130021,China | | LIU Yu-guang | School of Public Health, Jilin University, Changchun 130021,China | | NI Guan-ying | School of Public Health, Jilin University, Changchun 130021,China | | LIU Shu-zheng | School of Public Health, Jilin University, Changchun 130021,China |
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| Abstract:: |
| Objective To study the changes of reticulocyte micronucleus(MN-RET) from peripheral blood and polychromatic erythrocyte micronucleus(MN-PCE) from bone marrow in mice following exposure to X-rays in order to provide an experimental basis for exploring possible high-throughput radiation biodosimeter. Methods Male ICR mice were whole-body irradiated with 0, 0.5, 1, 2, 4 and 5 Gy at a dose rate of 0.488 Gy/min. MN-RET from peripheral blood was scored with FCM and MN-PCE from bone marrow was scored with manual microscopy at 24, 48 and 72 h post-irradiation. Results Both MN-RET and MN-PCE rates increased with doses in the range of 0-5 Gy at 24, 48 and 72 h after WBI. The dose-response relationship can be fit with linear equations (t=10.26-25.77, P<0.05). The correlation coefficients between MN-RET from peripheral blood and MN-PCE from bone marrow were highly significant (r=0.986-0.996, P<0.05). Conclusions In view of its simplicity, accuracy and high-throughput capacity, FCM scoring of peripheral blood MN-RET may be a candidate for radiation biodosimetry. More work should be carried out on human specimens to investigate this possibility. |
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