| LIU Jian-xiang,RUAN Jian-lei,TIAN Mei,et al.Effects of 60Co γ-ray partial radiation on chromosome aberration in human peripheral blood in vitro [J].Chinese Journal of Radiological Medicine and Protection,2011,31(1):38-40 |
| Effects of 60Co γ-ray partial radiation on chromosome aberration in human peripheral blood in vitro |
| Received:March 30, 2010 |
| DOI：10.3760/cma.j.issn.0254-5098.2011.01.010 |
| KeyWords:Partial irradiation Human peripheral blood Chromosome aberration |
| FundProject:卫生行业科研专项(200802018) |
| Author Name | Affiliation | | LIU Jian-xiang | National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | RUAN Jian-lei | National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | TIAN Mei | National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | PAN Yan | National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | SU Xu | National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China |
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| Abstract:: |
| Objective To investigate the effects of 60Co γ-ray partial radiation on chromosome aberration in human peripheral blood in vitro. Methods The samples of heparinized peripheral whole blood from 3 healthy persons were exposed to 60Co γ-rays at the doses between 0 and 8 Gy with the dose rate of 0.35 Gy/min at the temperature of 37 ℃, and then mixed with the unirradiated blood samples of the same donors by the ratio of 0.5 ∶1. 120 minutes later the samples of mixed blood underwent routine culture. Microscopy was used to observe the chromosome aberration double (centromere+centromere) and the biological dose was estimated thereby. Results The amounts of double centromere+centromere were increased along with the dose of irradiation in all groups. The estimated biological dose was higher than the 1/3 of the irradiation dose when the dose was between 0.5 to 2 Gy, and was close to the 1/3 of the irradiation dose when the dose was between 4 to 8 Gy. Conclusion Chromosome aberration can be used as a biomarker in estimation of uneven irradiation. |
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