| ZHU Rong-he,ZHUANG Qiang,CAI Fang-fang,et al.Relationship between intestinal permeability and expression of intestinal trefoil factor mRNA in mice after total body irradiation[J].Chinese Journal of Radiological Medicine and Protection,2011,31(1):29-32 |
| Relationship between intestinal permeability and expression of intestinal trefoil factor mRNA in mice after total body irradiation |
| Received:September 05, 2010 |
| DOI：10.3760/cma.j.issn.0254-5098.2011.01.008 |
| KeyWords:Total body irradiation (TBI) Intestinal permeability Intestinal trefoil factor (ITF) |
| FundProject:浙江省自然科学基金(Y207422);浙江省医药卫生科学研究基金(2007A137);温州市科技局科技合作项目(H20090010) |
| Author Name | Affiliation | | ZHU Rong-he | Department of Hematology, First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, China | | ZHUANG Qiang | Department of Hematology, First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, China | | CAI Fang-fang | Department of Hematology, First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, China | | YU Kang | Department of Hematology, First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, China | | JIANG Song-fu | Department of Hematology, First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, China |
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| Abstract:: |
| Objective To investigate the change of the intestinal permeability, the expression level of intestinal trefoil factor (ITF) mRNA and the relationship between them after total body irradiation (TBI), and explore the effect of TBI on the development of intestinal permeability and the expression level of ITF mRNA. Methods Twenty two BALB/c mice were randomly divided into 4 equal groups: 3 groups at 4, 8 and 12 d after TBI with the total dose of 8.0 Gy and the dose rate of 1.0 Gy/min respectively, and a control group. Lactulose (L) and mannitol (M) were perfused into the esophagus before the experiment and urine samples were collected. Liquid chromatography was used to measure the L/M excretion ratio in the urine samples collected 4, 8, and 12 days after the TBI. And then the mice were killed with their intestine were taken out. The expression of ITF mRNA in the jejunum tissue was detected by real-time fluorescence quantitative PCR. Results The urine L/M ratio levels of the groups 4, 8 and 12 days after TBI were (0.5092±0.0352), (0.7174±0.0116), and (0.7295±0.0533) respectively, all significantly higher than that of the control group ［(0.2908±0.0533), F=321.47,P<0.05］. The ITF mRNA expression levels of groups 4, 8 and 12 days after TBI were (0.78612±0.1428), (0.2521±0.1223), and (0.2306±0.0221) respectively, all significantly lower than that of the control group ［(1.3498±0.0476), F=235.71,P<0.05］. The urine L/M ratio was significantly negatively correlated with the expression of ITF mRNA in all TBI groups (r= -0.985, P<0.01). Conclusions The intestinal permeability increases and the expression level of ITF mRNA decreases after TBI. The urine L/M ratio is negatively correlated with the expression level of ITF mRNA after TBI. ITF is involved in protection against intestinal permeability induced by TBI. |
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